Protocols for making microscopy samples

Making PSF slides

• Use PS-Speck ORANGE beads, 170nm
• Using a small pipette tip, spread 2-3uL of beads around to the size of a 18x18 cover slip (do not draw a circle with permanent marker, as its drying seems to mess up the bead distribution)
• Let the slides air dry
• Drop ~5uL glycerol on the slide where the beads are
• Cover gently with cover slip, allow glycerol to spread to edge of cover slips
• Seal edges with nail polish