Difference between revisions of "Using CRISPRi to increase ethanol yield in E. coli MG1655"
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Noreen Lyell (Talk | contribs) (→Related modules) |
Noreen Lyell (Talk | contribs) (→Related modules) |
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==Related modules== | ==Related modules== | ||
− | #[[ |In silico cloning of pdCas9 construct]] | + | #[[In silico cloning of pdCas9 construct |In silico cloning of pdCas9 construct]] |
#*Students perform steps used in classic cloning using free, online DNA manipulation interface with laboratory exercises that can be completed in remote teaching format | #*Students perform steps used in classic cloning using free, online DNA manipulation interface with laboratory exercises that can be completed in remote teaching format | ||
#*Useful when timing doesn't allow for at-the-bench cloning laboratory exercise | #*Useful when timing doesn't allow for at-the-bench cloning laboratory exercise |
Revision as of 14:32, 2 August 2020
Overview
Laboratory exercises
Day 1:
Day 2:
Day 3:
Day 4:
Day 5:
Related modules
- In silico cloning of pdCas9 construct
- Students perform steps used in classic cloning using free, online DNA manipulation interface with laboratory exercises that can be completed in remote teaching format
- Useful when timing doesn't allow for at-the-bench cloning laboratory exercise
- Provides background details on methods used for plasmid construction that are important in laboratory exercises focused on the steps after cloning
- Steps for optional at-the-bench confirmation digest laboratory exercise included
- Optimizing sgRNA design to further increase ethanol yield (coming soon!)
- Students analyze datasets collected in previous semesters to design an optimized sgRNA sequence with laboratory exercises that can be completed in remote teaching format