Difference between revisions of "Talk:20.109(S16):Purify protein (Day6)"
From Course Wiki
(→OD562 absorbance measurements for protein concentration calculations) |
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|A | |A | ||
| − | | | + | |3.02 |
|- | |- | ||
|B | |B | ||
| − | | | + | |0.798 |
|- | |- | ||
|C | |C | ||
| − | | | + | |0.444 |
|- | |- | ||
|D | |D | ||
| − | | | + | |0.245 |
|- | |- | ||
|E | |E | ||
| − | | | + | |0.141 |
|- | |- | ||
|F | |F | ||
| − | | | + | |0.073 |
|- | |- | ||
|G | |G | ||
| − | | | + | |0.054 |
| − | + | ||
| − | + | ||
| − | + | ||
|- | |- | ||
|I | |I | ||
| − | | | + | |0.034 |
|- | |- | ||
|colspan=2| inverse pericam (IPC) | |colspan=2| inverse pericam (IPC) | ||
|- | |- | ||
|wild-type | |wild-type | ||
| − | | | + | |0.177 |
|- | |- | ||
|mutant | |mutant | ||
| − | | | + | |0.201 |
|} | |} | ||
Revision as of 22:28, 25 February 2016
OD562 absorbance measurements for protein concentration calculations
During the 1 hour incubation of Part4A use Microsoft Excel to prepare a graph of absorbance versus BSA concentration using the sample data here. You will need to use the trendline equation to calculate the protein concentration of your example elution data. You may also find this technical bulletin helpful.
T/R red team
| name | OD562 |
| standards | |
| A | 3.02 |
| B | 0.798 |
| C | 0.444 |
| D | 0.245 |
| E | 0.141 |
| F | 0.073 |
| G | 0.054 |
| I | 0.034 |
| inverse pericam (IPC) | |
| wild-type | 0.177 |
| mutant | 0.201 |
