Talk:20.109(S16):Flow cytometry and paper discussion (Day7)

From Course Wiki
Jump to: navigation, search

Files to download for analysis:

Instructor samples

Instructor FC sample key

Instructor FC plots

Instructor FC spreadsheet

Student samples

TR FC sample key

TR FC plots

TR FC spreadsheet

WF FC sample key

WF FC plots

WF FC spreadsheet

Tables for group data:

Report both the median and mean calculations for each replicate within the table according to the following format: median value, mean value

TR section

TR Red Team: (insert condition information here - enzymes and drug used)

Sample Normalized EGFP/mCherry value (replicate #1) Normalized EGFP/mCherry value (replicate #2)
M059K mCherry:EGFP 2.2 2.4
M059K mCherry:EGFP + drug 2.4 1.9
M059J mCherry:EGFP 3.0 2.9
M059K mCherry:damaged EGFP 0.11 0.13
M059K mCherry:damaged EGFP + drug 0.17 0.04
M059J mCherry:damaged EGFP 0 0


TR Orange Team: (insert condition information here - enzymes and drug used)

Sample Normalized EGFP/mCherry value (replicate #1) Normalized EGFP/mCherry value (replicate #2)
M059K mCherry:EGFP
M059K mCherry:EGFP + drug
M059J mCherry:EGFP
M059K mCherry:damaged EGFP
M059K mCherry:damaged EGFP + drug
M059J mCherry:damaged EGFP


TR Yellow Team: (insert condition information here - enzymes and drug used)

Sample Normalized EGFP/mCherry value (replicate #1) Normalized EGFP/mCherry value (replicate #2)
M059K mCherry:EGFP
M059K mCherry:EGFP + drug
M059J mCherry:EGFP
M059K mCherry:damaged EGFP
M059K mCherry:damaged EGFP + drug
M059J mCherry:damaged EGFP


TR Green Team: (insert condition information here - enzymes and drug used)

Sample Normalized EGFP/mCherry value (replicate #1) Normalized EGFP/mCherry value (replicate #2)
M059K mCherry:EGFP
M059K mCherry:EGFP + drug
M059J mCherry:EGFP
M059K mCherry:damaged EGFP
M059K mCherry:damaged EGFP + drug
M059J mCherry:damaged EGFP


TR Blue Team: (insert condition information here - enzymes and drug used)

Sample Normalized EGFP/mCherry value (replicate #1) Normalized EGFP/mCherry value (replicate #2)
M059K mCherry:EGFP
M059K mCherry:EGFP + drug
M059J mCherry:EGFP
M059K mCherry:damaged EGFP
M059K mCherry:damaged EGFP + drug
M059J mCherry:damaged EGFP


TR Pink Team: EcoRI-BglII DMNB

Sample Normalized EGFP/mCherry value (replicate #1) Normalized EGFP/mCherry value (replicate #2)
M059K mCherry:EGFP 1.93, 2.21 2.97, 2.39
M059K mCherry:EGFP + drug 2.20, 2.38 0.98, 1.92
M059J mCherry:EGFP 2.92, 2,96 2.79, 2.90
M059K mCherry:damaged EGFP 0.05, 0.11 0.11, 0.13
M059K mCherry:damaged EGFP + drug 0.27, 0.17 0.05, 0.03
M059J mCherry:damaged EGFP 0, 0 0,0


TR Purple Team: (insert condition information here - enzymes and drug used)

Sample Normalized EGFP/mCherry value (replicate #1) Normalized EGFP/mCherry value (replicate #2)
M059K mCherry:EGFP
M059K mCherry:EGFP + drug
M059J mCherry:EGFP
M059K mCherry:damaged EGFP
M059K mCherry:damaged EGFP + drug
M059J mCherry:damaged EGFP

WF section

WF Red Team: (insert condition information here - enzymes and drug used)

Sample Normalized EGFP/mCherry value (replicate #1) Normalized EGFP/mCherry value (replicate #2)
M059K mCherry:EGFP
M059K mCherry:EGFP + drug
M059J mCherry:EGFP
M059K mCherry:damaged EGFP
M059K mCherry:damaged EGFP + drug
M059J mCherry:damaged EGFP


WF Orange Team: (insert condition information here - enzymes and drug used)

Sample Normalized EGFP/mCherry value (replicate #1) Normalized EGFP/mCherry value (replicate #2)
M059K mCherry:EGFP
M059K mCherry:EGFP + drug
M059J mCherry:EGFP
M059K mCherry:damaged EGFP
M059K mCherry:damaged EGFP + drug
M059J mCherry:damaged EGFP


WF Blue Team: (insert condition information here - enzymes and drug used)

Sample Normalized EGFP/mCherry value (replicate #1) Normalized EGFP/mCherry value (replicate #2)
M059K mCherry:EGFP
M059K mCherry:EGFP + drug
M059J mCherry:EGFP
M059K mCherry:damaged EGFP
M059K mCherry:damaged EGFP + drug
M059J mCherry:damaged EGFP


WF Pink Team: (insert condition information here - enzymes and drug used)

Sample Normalized EGFP/mCherry value (replicate #1) Normalized EGFP/mCherry value (replicate #2)
M059K mCherry:EGFP
M059K mCherry:EGFP + drug
M059J mCherry:EGFP
M059K mCherry:damaged EGFP
M059K mCherry:damaged EGFP + drug
M059J mCherry:damaged EGFP


WF Purple Team: (insert condition information here - enzymes and drug used)

Sample Normalized EGFP/mCherry value (replicate #1) Normalized EGFP/mCherry value (replicate #2)
M059K mCherry:EGFP
M059K mCherry:EGFP + drug
M059J mCherry:EGFP
M059K mCherry:damaged EGFP
M059K mCherry:damaged EGFP + drug
M059J mCherry:damaged EGFP