Difference between revisions of "Talk:20.109(S16):Flow cytometry and paper discussion (Day7)"

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TR Yellow Team: '''(insert condition information here - enzymes and drug used)'''
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TR Yellow Team: '''(BglII PstI DMNB)'''
 
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|M059K mCherry:EGFP
 
|M059K mCherry:EGFP
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|2.4152710957457
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|2.21080310584731
 
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|-
 
|M059K mCherry:EGFP + drug
 
|M059K mCherry:EGFP + drug
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+
|2.21472333442984
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|2.38114333521407
 
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|-
 
|M059J mCherry:EGFP
 
|M059J mCherry:EGFP
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|3.20341824288404
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|2.59325959019731
 
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|-
 
|M059K mCherry:damaged EGFP
 
|M059K mCherry:damaged EGFP
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|0.183679950306649
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|0.180907303326043
 
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|-
 
|M059K mCherry:damaged EGFP + drug
 
|M059K mCherry:damaged EGFP + drug
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|0.187012607850519
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|0.025193176202374
 
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|-
 
|M059J mCherry:damaged EGFP
 
|M059J mCherry:damaged EGFP
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|0
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|0
 
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Revision as of 19:17, 5 April 2016

Files to download for analysis:

Instructor samples

Instructor FC sample key

Instructor FC plots

Instructor FC spreadsheet

Student samples

TR FC sample key

TR FC plots

TR FC spreadsheet

WF FC sample key

WF FC plots

WF FC spreadsheet

Tables for group data:

Report both the median and mean calculations for each replicate within the table according to the following format: median value, mean value

TR section

TR Red Team: (insert condition information here - enzymes and drug used)

Sample Normalized EGFP/mCherry value (replicate #1) Normalized EGFP/mCherry value (replicate #2)
M059K mCherry:EGFP 2.2, 1.9 2.4, 3.0
M059K mCherry:EGFP + drug 2.4, 2.2 1.9, 1.0
M059J mCherry:EGFP 3.0, 3.0 2.9, 2.8
M059K mCherry:damaged EGFP 0.11, 0.05 0.13, 0.12
M059K mCherry:damaged EGFP + drug 0.17, 0.27 0.04, 0.05
M059J mCherry:damaged EGFP 0, 0 0, 0


TR Orange Team: (insert condition information here - enzymes and drug used)

Sample Normalized EGFP/mCherry value (replicate #1) Normalized EGFP/mCherry value (replicate #2)
M059K mCherry:EGFP 2.09, 1.77 2.54, 2.60
M059K mCherry:EGFP + drug 3.04, 1.95 2.58, 3.49
M059J mCherry:EGFP 4.11, 2.09 2.70, 1.65
M059K mCherry:damaged EGFP 0.24, 0.21 0.21, 0.13
M059K mCherry:damaged EGFP + drug 0.07, 0.08 0.18, 0.21
M059J mCherry:damaged EGFP 0.01, 0.03 0.04, 0.02


TR Yellow Team: (BglII PstI DMNB)

Sample Normalized EGFP/mCherry value (replicate #1) Normalized EGFP/mCherry value (replicate #2)
M059K mCherry:EGFP 2.4152710957457 2.21080310584731
M059K mCherry:EGFP + drug 2.21472333442984 2.38114333521407
M059J mCherry:EGFP 3.20341824288404 2.59325959019731
M059K mCherry:damaged EGFP 0.183679950306649 0.180907303326043
M059K mCherry:damaged EGFP + drug 0.187012607850519 0.025193176202374
M059J mCherry:damaged EGFP 0 0


TR Green Team: (insert condition information here - enzymes and drug used)

Sample Normalized EGFP/mCherry value (replicate #1) Normalized EGFP/mCherry value (replicate #2)
M059K mCherry:EGFP
M059K mCherry:EGFP + drug
M059J mCherry:EGFP
M059K mCherry:damaged EGFP
M059K mCherry:damaged EGFP + drug
M059J mCherry:damaged EGFP


TR Blue Team: (EcoRI + BglII - Loperamide)

Sample Normalized EGFP/mCherry value (replicate #1) Normalized EGFP/mCherry value (replicate #2)
M059K mCherry:EGFP 2.252,2.358 2.422,2.271
M059K mCherry:EGFP + drug 2.088,2.223 1.758,2.218
M059J mCherry:EGFP 2.847,3.344 2.560,3.024
M059K mCherry:damaged EGFP 0.104,0.085 0.146,0.204
M059K mCherry:damaged EGFP + drug 0.133,0.109 0.087,0.114
M059J mCherry:damaged EGFP 0.005,0.002 0.079,0.037


TR Pink Team: EcoRI-BglII DMNB

Sample Normalized EGFP/mCherry value (replicate #1) Normalized EGFP/mCherry value (replicate #2)
M059K mCherry:EGFP 1.93, 2.21 2.97, 2.39
M059K mCherry:EGFP + drug 2.20, 2.38 0.98, 1.92
M059J mCherry:EGFP 2.92, 2,96 2.79, 2.90
M059K mCherry:damaged EGFP 0.05, 0.11 0.11, 0.13
M059K mCherry:damaged EGFP + drug 0.27, 0.17 0.05, 0.03
M059J mCherry:damaged EGFP 0, 0 0,0


TR Purple Team: (insert condition information here - enzymes and drug used)

Sample Normalized EGFP/mCherry value (replicate #1) Normalized EGFP/mCherry value (replicate #2)
M059K mCherry:EGFP
M059K mCherry:EGFP + drug
M059J mCherry:EGFP
M059K mCherry:damaged EGFP
M059K mCherry:damaged EGFP + drug
M059J mCherry:damaged EGFP

WF section

WF Red Team: (insert condition information here - enzymes and drug used)

Sample Normalized EGFP/mCherry value (replicate #1) Normalized EGFP/mCherry value (replicate #2)
M059K mCherry:EGFP
M059K mCherry:EGFP + drug
M059J mCherry:EGFP
M059K mCherry:damaged EGFP
M059K mCherry:damaged EGFP + drug
M059J mCherry:damaged EGFP


WF Orange Team: (insert condition information here - enzymes and drug used)

Sample Normalized EGFP/mCherry value (replicate #1) Normalized EGFP/mCherry value (replicate #2)
M059K mCherry:EGFP
M059K mCherry:EGFP + drug
M059J mCherry:EGFP
M059K mCherry:damaged EGFP
M059K mCherry:damaged EGFP + drug
M059J mCherry:damaged EGFP


WF Blue Team: (insert condition information here - enzymes and drug used)

Sample Normalized EGFP/mCherry value (replicate #1) Normalized EGFP/mCherry value (replicate #2)
M059K mCherry:EGFP
M059K mCherry:EGFP + drug
M059J mCherry:EGFP
M059K mCherry:damaged EGFP
M059K mCherry:damaged EGFP + drug
M059J mCherry:damaged EGFP


WF Pink Team: (insert condition information here - enzymes and drug used)

Sample Normalized EGFP/mCherry value (replicate #1) Normalized EGFP/mCherry value (replicate #2)
M059K mCherry:EGFP
M059K mCherry:EGFP + drug
M059J mCherry:EGFP
M059K mCherry:damaged EGFP
M059K mCherry:damaged EGFP + drug
M059J mCherry:damaged EGFP


WF Purple Team: (insert condition information here - enzymes and drug used)

Sample Normalized EGFP/mCherry value (replicate #1) Normalized EGFP/mCherry value (replicate #2)
M059K mCherry:EGFP
M059K mCherry:EGFP + drug
M059J mCherry:EGFP
M059K mCherry:damaged EGFP
M059K mCherry:damaged EGFP + drug
M059J mCherry:damaged EGFP
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