Difference between revisions of "Talk:20.109(S16):Flow cytometry and paper discussion (Day7)"

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(→‎Files to download for analysis:)
(→‎WF section)
 
(21 intermediate revisions by 11 users not shown)
Line 7: Line 7:
 
[[Media:Sp16 Instructor FC plots 160404.pdf| Instructor FC plots]]
 
[[Media:Sp16 Instructor FC plots 160404.pdf| Instructor FC plots]]
  
−
[[Media:Sp16 Instructor FC Spreadsheet 160404.xlsx| Instructor FC Spreadsheet]]
+
[[Media:Sp16 Instructor FC Spreadsheet 160405.xlsx| Instructor FC spreadsheet]]
  
 
====Student samples====
 
====Student samples====
Line 28: Line 28:
  
 
====TR section====
 
====TR section====
−
TR Red Team: '''(insert condition information here - enzymes and drug used)'''
+
TR Red Team: '''(EcoRI, BGlII, DMNB)'''
 
<center>
 
<center>
 
{| border="1"
 
{| border="1"
Line 36: Line 36:
 
|-
 
|-
 
|M059K mCherry:EGFP
 
|M059K mCherry:EGFP
−
|
+
|2.2, 1.9
−
|
+
|2.4, 3.0
 
|-
 
|-
 
|M059K mCherry:EGFP + drug
 
|M059K mCherry:EGFP + drug
−
|
+
|2.4, 2.2
−
|
+
|1.9, 1.0
 
|-
 
|-
 
|M059J mCherry:EGFP
 
|M059J mCherry:EGFP
−
|
+
|3.0, 3.0
−
|
+
|2.9, 2.8
 
|-
 
|-
 
|M059K mCherry:damaged EGFP
 
|M059K mCherry:damaged EGFP
−
|
+
|0.11, 0.05
−
|
+
|0.13, 0.12
 
|-
 
|-
 
|M059K mCherry:damaged EGFP + drug
 
|M059K mCherry:damaged EGFP + drug
−
|
+
|0.17, 0.27
−
|
+
|0.04, 0.05
 
|-
 
|-
 
|M059J mCherry:damaged EGFP
 
|M059J mCherry:damaged EGFP
−
|
+
|0, 0
−
|
+
|0, 0
 
|-
 
|-
 
|}
 
|}
Line 63: Line 63:
  
  
−
TR Orange Team: '''(insert condition information here - enzymes and drug used)'''
+
TR Orange Team: '''(PmeI and DMNB)'''
 
<center>
 
<center>
 
{| border="1"
 
{| border="1"
Line 71: Line 71:
 
|-
 
|-
 
|M059K mCherry:EGFP
 
|M059K mCherry:EGFP
−
|
+
|2.09, 1.77
−
|
+
|2.54, 2.60
 
|-
 
|-
 
|M059K mCherry:EGFP + drug
 
|M059K mCherry:EGFP + drug
−
|
+
|3.04, 1.95
−
|
+
|2.58, 3.49
 
|-
 
|-
 
|M059J mCherry:EGFP
 
|M059J mCherry:EGFP
−
|
+
|4.11, 2.09
−
|
+
|2.70, 1.65
 
|-
 
|-
 
|M059K mCherry:damaged EGFP
 
|M059K mCherry:damaged EGFP
−
|
+
|0.24, 0.21
−
|
+
|0.21, 0.13
 
|-
 
|-
 
|M059K mCherry:damaged EGFP + drug
 
|M059K mCherry:damaged EGFP + drug
−
|
+
|0.07, 0.08
−
|
+
|0.18, 0.21
 
|-
 
|-
 
|M059J mCherry:damaged EGFP
 
|M059J mCherry:damaged EGFP
−
|
+
|0.01, 0.03
−
|
+
|0.04, 0.02
 
|-
 
|-
 
|}
 
|}
Line 98: Line 98:
  
  
−
TR Yellow Team: '''(insert condition information here - enzymes and drug used)'''
+
TR Yellow Team: '''(BglII PstI DMNB)'''
 
<center>
 
<center>
 
{| border="1"
 
{| border="1"
Line 106: Line 106:
 
|-
 
|-
 
|M059K mCherry:EGFP
 
|M059K mCherry:EGFP
−
|
+
|2.4, 2.4
−
|
+
|2.2, 2.6
 
|-
 
|-
 
|M059K mCherry:EGFP + drug
 
|M059K mCherry:EGFP + drug
−
|
+
|2.2, 2.2
−
|
+
|2.4, 2.8
 
|-
 
|-
 
|M059J mCherry:EGFP
 
|M059J mCherry:EGFP
−
|
+
|3.2, 3.0
−
|
+
|2.6, 2.2
 
|-
 
|-
 
|M059K mCherry:damaged EGFP
 
|M059K mCherry:damaged EGFP
−
|
+
|0.18, 0.15
−
|
+
|0.18, .18
 
|-
 
|-
 
|M059K mCherry:damaged EGFP + drug
 
|M059K mCherry:damaged EGFP + drug
−
|
+
|0.19, 0.61
−
|
+
|0.025, 0.030
 
|-
 
|-
 
|M059J mCherry:damaged EGFP
 
|M059J mCherry:damaged EGFP
−
|
+
|0
−
|
+
|0
 
|-
 
|-
 
|}
 
|}
Line 133: Line 133:
  
  
−
TR Green Team: '''(insert condition information here - enzymes and drug used)'''
+
TR Green Team: '''(PmeI and Loperamide)'''
 
<center>
 
<center>
 
{| border="1"
 
{| border="1"
Line 141: Line 141:
 
|-
 
|-
 
|M059K mCherry:EGFP
 
|M059K mCherry:EGFP
−
|
+
|3.03, 2.26
−
|
+
|2.44, 2.34
 
|-
 
|-
 
|M059K mCherry:EGFP + drug
 
|M059K mCherry:EGFP + drug
−
|
+
|2.88, 2.07
−
|
+
|3.05, 2.20
 
|-
 
|-
 
|M059J mCherry:EGFP
 
|M059J mCherry:EGFP
−
|
+
|2.07, 3.01
−
|
+
|1.75, 2.12
 
|-
 
|-
 
|M059K mCherry:damaged EGFP
 
|M059K mCherry:damaged EGFP
−
|
+
|0.25, 0.24
−
|
+
|0.19, 0.34
 
|-
 
|-
 
|M059K mCherry:damaged EGFP + drug
 
|M059K mCherry:damaged EGFP + drug
−
|
+
|0.12, 0.31
−
|
+
|0.16, 0.29
 
|-
 
|-
 
|M059J mCherry:damaged EGFP
 
|M059J mCherry:damaged EGFP
−
|
+
|0.05, 0.02
−
|
+
|0, 0
 
|-
 
|-
 
|}
 
|}
Line 168: Line 168:
  
  
−
TR Blue Team: '''(insert condition information here - enzymes and drug used)'''
+
TR Blue Team: '''(EcoRI + BglII - Loperamide)'''
 
<center>
 
<center>
 
{| border="1"
 
{| border="1"
Line 176: Line 176:
 
|-
 
|-
 
|M059K mCherry:EGFP
 
|M059K mCherry:EGFP
−
|
+
|2.252,2.358
−
|
+
|2.422,2.271
 
|-
 
|-
 
|M059K mCherry:EGFP + drug
 
|M059K mCherry:EGFP + drug
−
|
+
|2.088,2.223
−
|
+
|1.758,2.218
 
|-
 
|-
 
|M059J mCherry:EGFP
 
|M059J mCherry:EGFP
−
|
+
|2.847,3.344
−
|
+
|2.560,3.024
 
|-
 
|-
 
|M059K mCherry:damaged EGFP
 
|M059K mCherry:damaged EGFP
−
|
+
|0.104,0.085
−
|
+
|0.146,0.204
 
|-
 
|-
 
|M059K mCherry:damaged EGFP + drug
 
|M059K mCherry:damaged EGFP + drug
−
|
+
|0.133,0.109
−
|
+
|0.087,0.114
 
|-
 
|-
 
|M059J mCherry:damaged EGFP
 
|M059J mCherry:damaged EGFP
−
|
+
|0.005,0.002
−
|
+
|0.079,0.037
 
|-
 
|-
 
|}
 
|}
Line 203: Line 203:
  
  
−
TR Pink Team: '''(insert condition information here - enzymes and drug used)'''
+
TR Pink Team: '''EcoRI-BglII DMNB'''
 
<center>
 
<center>
 
{| border="1"
 
{| border="1"
Line 211: Line 211:
 
|-
 
|-
 
|M059K mCherry:EGFP
 
|M059K mCherry:EGFP
−
|
+
|1.93, 2.21
−
|
+
|2.97, 2.39
 
|-
 
|-
 
|M059K mCherry:EGFP + drug
 
|M059K mCherry:EGFP + drug
−
|
+
|2.20, 2.38
−
|
+
|0.98, 1.92
 
|-
 
|-
 
|M059J mCherry:EGFP
 
|M059J mCherry:EGFP
−
|
+
|2.92, 2,96
−
|
+
|2.79, 2.90
 
|-
 
|-
 
|M059K mCherry:damaged EGFP
 
|M059K mCherry:damaged EGFP
−
|
+
|0.05, 0.11
−
|
+
|0.11, 0.13
 
|-
 
|-
 
|M059K mCherry:damaged EGFP + drug
 
|M059K mCherry:damaged EGFP + drug
−
|
+
|0.27, 0.17
−
|
+
|0.05, 0.03
 
|-
 
|-
 
|M059J mCherry:damaged EGFP
 
|M059J mCherry:damaged EGFP
−
|
+
|0, 0
−
|
+
|0,0
 
|-
 
|-
 
|}
 
|}
Line 238: Line 238:
  
  
−
TR Purple Team: '''(insert condition information here - enzymes and drug used)'''
+
TR Purple Team: '''PmeI DMNB'''
 
<center>
 
<center>
 
{| border="1"
 
{| border="1"
Line 246: Line 246:
 
|-
 
|-
 
|M059K mCherry:EGFP
 
|M059K mCherry:EGFP
−
|
+
|1.772724608, 2.085453242
−
|
+
|2.600281239, 2.537742473
 
|-
 
|-
 
|M059K mCherry:EGFP + drug
 
|M059K mCherry:EGFP + drug
−
|
+
|1.948901491, 3.044622474
−
|
+
|3.487826161, 2.579098259
 
|-
 
|-
 
|M059J mCherry:EGFP
 
|M059J mCherry:EGFP
−
|
+
|2.092658688, 4.11618439
−
|
+
|1.651441549, 2.700436438
 
|-
 
|-
 
|M059K mCherry:damaged EGFP
 
|M059K mCherry:damaged EGFP
−
|
+
|0.207545736, 0.240272044
−
|
+
|0.133947935, 0.214451077
 
|-
 
|-
 
|M059K mCherry:damaged EGFP + drug
 
|M059K mCherry:damaged EGFP + drug
−
|
+
|0.084415562, 0.074686032
−
|
+
|0.208750497, 0.175961141
 
|-
 
|-
 
|M059J mCherry:damaged EGFP
 
|M059J mCherry:damaged EGFP
−
|
+
|0.026551063, 0.011684889
−
|
+
|0.015829439, 0.04141871
 
|-
 
|-
 
|}
 
|}
Line 274: Line 274:
 
====WF section====
 
====WF section====
  
−
WF Red Team: '''(insert condition information here - enzymes and drug used)'''
+
WF Red Team: '''BglII & PstI, Loperamide'''
 
<center>
 
<center>
 
{| border="1"
 
{| border="1"
Line 282: Line 282:
 
|-
 
|-
 
|M059K mCherry:EGFP
 
|M059K mCherry:EGFP
−
|
+
|1.9, 2.2
−
|
+
|2.25, 2.28
 
|-
 
|-
 
|M059K mCherry:EGFP + drug
 
|M059K mCherry:EGFP + drug
−
|
+
|2.88, 2.59
−
|
+
|2.77, 2.25
 
|-
 
|-
 
|M059J mCherry:EGFP
 
|M059J mCherry:EGFP
−
|
+
|2.97, 3.75
−
|
+
|1.71, 2.4
 
|-
 
|-
 
|M059K mCherry:damaged EGFP
 
|M059K mCherry:damaged EGFP
−
|
+
|0.11, 0.12
−
|
+
|0.1, 0.14
 
|-
 
|-
 
|M059K mCherry:damaged EGFP + drug
 
|M059K mCherry:damaged EGFP + drug
−
|
+
|0.09, 0.09
−
|
+
|0.07, 0.06
 
|-
 
|-
 
|M059J mCherry:damaged EGFP
 
|M059J mCherry:damaged EGFP
−
|
+
|0.04, 0.02
−
|
+
|0.02, 0.01
 
|-
 
|-
 
|}
 
|}
Line 309: Line 309:
  
  
−
WF Orange Team: '''(insert condition information here - enzymes and drug used)'''
+
WF Orange Team: ''' ECORI + BglII - Loperamide'''
 
<center>
 
<center>
 
{| border="1"
 
{| border="1"
Line 317: Line 317:
 
|-
 
|-
 
|M059K mCherry:EGFP
 
|M059K mCherry:EGFP
−
|
+
|2.25, 2.36
−
|
+
|2.42, 2.27
 
|-
 
|-
 
|M059K mCherry:EGFP + drug
 
|M059K mCherry:EGFP + drug
−
|
+
|2.09, 2.22
−
|
+
|1.76, 2.22
 
|-
 
|-
 
|M059J mCherry:EGFP
 
|M059J mCherry:EGFP
−
|
+
|2.85, 3.34
−
|
+
|2.56, 3.02
 
|-
 
|-
 
|M059K mCherry:damaged EGFP
 
|M059K mCherry:damaged EGFP
−
|
+
|0.10, 0.09
−
|
+
|0.15, 0.20
 
|-
 
|-
 
|M059K mCherry:damaged EGFP + drug
 
|M059K mCherry:damaged EGFP + drug
−
|
+
|0.13, 0.11
−
|
+
|0.09, 0.11
 
|-
 
|-
 
|M059J mCherry:damaged EGFP
 
|M059J mCherry:damaged EGFP
−
|
+
|0.01, 0.00
−
|
+
|0.08, 0.04
 
|-
 
|-
 
|}
 
|}
Line 344: Line 344:
  
  
−
WF Blue Team: '''(insert condition information here - enzymes and drug used)'''
+
WF Blue Team: '''(PmeI Loperamide)'''
 
<center>
 
<center>
 
{| border="1"
 
{| border="1"
Line 352: Line 352:
 
|-
 
|-
 
|M059K mCherry:EGFP
 
|M059K mCherry:EGFP
−
|
+
|3.02, 2.25
−
|
+
|2.43, 2.34
 
|-
 
|-
 
|M059K mCherry:EGFP + drug
 
|M059K mCherry:EGFP + drug
−
|
+
|2.88, 2.07
−
|
+
|3.05, 2.20
 
|-
 
|-
 
|M059J mCherry:EGFP
 
|M059J mCherry:EGFP
−
|
+
|2.06, 3.1
−
|
+
|1.75,2.12
 
|-
 
|-
 
|M059K mCherry:damaged EGFP
 
|M059K mCherry:damaged EGFP
−
|
+
|0.254, 0.241
−
|
+
|0.19, 0.33
 
|-
 
|-
 
|M059K mCherry:damaged EGFP + drug
 
|M059K mCherry:damaged EGFP + drug
−
|
+
|0.12, 0.31
−
|
+
|0.16, 0.29
 
|-
 
|-
 
|M059J mCherry:damaged EGFP
 
|M059J mCherry:damaged EGFP
−
|
+
|0.05, 0.02
−
|
+
|0, 0
 
|-
 
|-
 
|}
 
|}
Line 379: Line 379:
  
  
−
WF Pink Team: '''(insert condition information here - enzymes and drug used)'''
+
WF Pink Team: '''(PstI & BglII, Loperamide)'''
 
<center>
 
<center>
 
{| border="1"
 
{| border="1"
Line 387: Line 387:
 
|-
 
|-
 
|M059K mCherry:EGFP
 
|M059K mCherry:EGFP
−
|
+
|1.90, 2.20
−
|
+
|2.25, 2.28
 
|-
 
|-
 
|M059K mCherry:EGFP + drug
 
|M059K mCherry:EGFP + drug
−
|
+
|2.88, 2.59
−
|
+
|2.77, 2.25
 
|-
 
|-
 
|M059J mCherry:EGFP
 
|M059J mCherry:EGFP
−
|
+
|2.97, 3.75
−
|
+
|1.71, 2.40
 
|-
 
|-
 
|M059K mCherry:damaged EGFP
 
|M059K mCherry:damaged EGFP
−
|
+
|0.11, 0.12
−
|
+
|0.10, 0.14
 
|-
 
|-
 
|M059K mCherry:damaged EGFP + drug
 
|M059K mCherry:damaged EGFP + drug
−
|
+
|0.09, 0.09
−
|
+
|0.07, 0.06
 
|-
 
|-
 
|M059J mCherry:damaged EGFP
 
|M059J mCherry:damaged EGFP
−
|
+
|0.04, 0.02
−
|
+
|0.02, 0.01
 
|-
 
|-
 
|}
 
|}
Line 414: Line 414:
  
  
−
WF Purple Team: '''(insert condition information here - enzymes and drug used)'''
+
WF Purple Team: '''PstI-BglII, DMNB'''
 
<center>
 
<center>
 
{| border="1"
 
{| border="1"
Line 422: Line 422:
 
|-
 
|-
 
|M059K mCherry:EGFP
 
|M059K mCherry:EGFP
−
|
+
|2.42, 2.43
−
|
+
|2.21, 2.58
 
|-
 
|-
 
|M059K mCherry:EGFP + drug
 
|M059K mCherry:EGFP + drug
−
|
+
|2.21, 2.20
−
|
+
|2.38, 2.79
 
|-
 
|-
 
|M059J mCherry:EGFP
 
|M059J mCherry:EGFP
−
|
+
|3.20, 2.99
−
|
+
|2.59, 2.20
 
|-
 
|-
 
|M059K mCherry:damaged EGFP
 
|M059K mCherry:damaged EGFP
−
|
+
|0.18, 0.15
−
|
+
|0.18, 0.18
 
|-
 
|-
 
|M059K mCherry:damaged EGFP + drug
 
|M059K mCherry:damaged EGFP + drug
−
|
+
|0.19, 0.61
−
|
+
|0.03, 0.03
 
|-
 
|-
 
|M059J mCherry:damaged EGFP
 
|M059J mCherry:damaged EGFP
−
|
+
|0, 0
−
|
+
|0, 0
 
|-
 
|-
 
|}
 
|}
 
</center>
 
</center>

Latest revision as of 04:54, 13 April 2016

Files to download for analysis:

Instructor samples

Instructor FC sample key

Instructor FC plots

Instructor FC spreadsheet

Student samples

TR FC sample key

TR FC plots

TR FC spreadsheet

WF FC sample key

WF FC plots

WF FC spreadsheet

Tables for group data:

Report both the median and mean calculations for each replicate within the table according to the following format: median value, mean value

TR section

TR Red Team: (EcoRI, BGlII, DMNB)

Sample Normalized EGFP/mCherry value (replicate #1) Normalized EGFP/mCherry value (replicate #2)
M059K mCherry:EGFP 2.2, 1.9 2.4, 3.0
M059K mCherry:EGFP + drug 2.4, 2.2 1.9, 1.0
M059J mCherry:EGFP 3.0, 3.0 2.9, 2.8
M059K mCherry:damaged EGFP 0.11, 0.05 0.13, 0.12
M059K mCherry:damaged EGFP + drug 0.17, 0.27 0.04, 0.05
M059J mCherry:damaged EGFP 0, 0 0, 0


TR Orange Team: (PmeI and DMNB)

Sample Normalized EGFP/mCherry value (replicate #1) Normalized EGFP/mCherry value (replicate #2)
M059K mCherry:EGFP 2.09, 1.77 2.54, 2.60
M059K mCherry:EGFP + drug 3.04, 1.95 2.58, 3.49
M059J mCherry:EGFP 4.11, 2.09 2.70, 1.65
M059K mCherry:damaged EGFP 0.24, 0.21 0.21, 0.13
M059K mCherry:damaged EGFP + drug 0.07, 0.08 0.18, 0.21
M059J mCherry:damaged EGFP 0.01, 0.03 0.04, 0.02


TR Yellow Team: (BglII PstI DMNB)

Sample Normalized EGFP/mCherry value (replicate #1) Normalized EGFP/mCherry value (replicate #2)
M059K mCherry:EGFP 2.4, 2.4 2.2, 2.6
M059K mCherry:EGFP + drug 2.2, 2.2 2.4, 2.8
M059J mCherry:EGFP 3.2, 3.0 2.6, 2.2
M059K mCherry:damaged EGFP 0.18, 0.15 0.18, .18
M059K mCherry:damaged EGFP + drug 0.19, 0.61 0.025, 0.030
M059J mCherry:damaged EGFP 0 0


TR Green Team: (PmeI and Loperamide)

Sample Normalized EGFP/mCherry value (replicate #1) Normalized EGFP/mCherry value (replicate #2)
M059K mCherry:EGFP 3.03, 2.26 2.44, 2.34
M059K mCherry:EGFP + drug 2.88, 2.07 3.05, 2.20
M059J mCherry:EGFP 2.07, 3.01 1.75, 2.12
M059K mCherry:damaged EGFP 0.25, 0.24 0.19, 0.34
M059K mCherry:damaged EGFP + drug 0.12, 0.31 0.16, 0.29
M059J mCherry:damaged EGFP 0.05, 0.02 0, 0


TR Blue Team: (EcoRI + BglII - Loperamide)

Sample Normalized EGFP/mCherry value (replicate #1) Normalized EGFP/mCherry value (replicate #2)
M059K mCherry:EGFP 2.252,2.358 2.422,2.271
M059K mCherry:EGFP + drug 2.088,2.223 1.758,2.218
M059J mCherry:EGFP 2.847,3.344 2.560,3.024
M059K mCherry:damaged EGFP 0.104,0.085 0.146,0.204
M059K mCherry:damaged EGFP + drug 0.133,0.109 0.087,0.114
M059J mCherry:damaged EGFP 0.005,0.002 0.079,0.037


TR Pink Team: EcoRI-BglII DMNB

Sample Normalized EGFP/mCherry value (replicate #1) Normalized EGFP/mCherry value (replicate #2)
M059K mCherry:EGFP 1.93, 2.21 2.97, 2.39
M059K mCherry:EGFP + drug 2.20, 2.38 0.98, 1.92
M059J mCherry:EGFP 2.92, 2,96 2.79, 2.90
M059K mCherry:damaged EGFP 0.05, 0.11 0.11, 0.13
M059K mCherry:damaged EGFP + drug 0.27, 0.17 0.05, 0.03
M059J mCherry:damaged EGFP 0, 0 0,0


TR Purple Team: PmeI DMNB

Sample Normalized EGFP/mCherry value (replicate #1) Normalized EGFP/mCherry value (replicate #2)
M059K mCherry:EGFP 1.772724608, 2.085453242 2.600281239, 2.537742473
M059K mCherry:EGFP + drug 1.948901491, 3.044622474 3.487826161, 2.579098259
M059J mCherry:EGFP 2.092658688, 4.11618439 1.651441549, 2.700436438
M059K mCherry:damaged EGFP 0.207545736, 0.240272044 0.133947935, 0.214451077
M059K mCherry:damaged EGFP + drug 0.084415562, 0.074686032 0.208750497, 0.175961141
M059J mCherry:damaged EGFP 0.026551063, 0.011684889 0.015829439, 0.04141871

WF section

WF Red Team: BglII & PstI, Loperamide

Sample Normalized EGFP/mCherry value (replicate #1) Normalized EGFP/mCherry value (replicate #2)
M059K mCherry:EGFP 1.9, 2.2 2.25, 2.28
M059K mCherry:EGFP + drug 2.88, 2.59 2.77, 2.25
M059J mCherry:EGFP 2.97, 3.75 1.71, 2.4
M059K mCherry:damaged EGFP 0.11, 0.12 0.1, 0.14
M059K mCherry:damaged EGFP + drug 0.09, 0.09 0.07, 0.06
M059J mCherry:damaged EGFP 0.04, 0.02 0.02, 0.01


WF Orange Team: ECORI + BglII - Loperamide

Sample Normalized EGFP/mCherry value (replicate #1) Normalized EGFP/mCherry value (replicate #2)
M059K mCherry:EGFP 2.25, 2.36 2.42, 2.27
M059K mCherry:EGFP + drug 2.09, 2.22 1.76, 2.22
M059J mCherry:EGFP 2.85, 3.34 2.56, 3.02
M059K mCherry:damaged EGFP 0.10, 0.09 0.15, 0.20
M059K mCherry:damaged EGFP + drug 0.13, 0.11 0.09, 0.11
M059J mCherry:damaged EGFP 0.01, 0.00 0.08, 0.04


WF Blue Team: (PmeI Loperamide)

Sample Normalized EGFP/mCherry value (replicate #1) Normalized EGFP/mCherry value (replicate #2)
M059K mCherry:EGFP 3.02, 2.25 2.43, 2.34
M059K mCherry:EGFP + drug 2.88, 2.07 3.05, 2.20
M059J mCherry:EGFP 2.06, 3.1 1.75,2.12
M059K mCherry:damaged EGFP 0.254, 0.241 0.19, 0.33
M059K mCherry:damaged EGFP + drug 0.12, 0.31 0.16, 0.29
M059J mCherry:damaged EGFP 0.05, 0.02 0, 0


WF Pink Team: (PstI & BglII, Loperamide)

Sample Normalized EGFP/mCherry value (replicate #1) Normalized EGFP/mCherry value (replicate #2)
M059K mCherry:EGFP 1.90, 2.20 2.25, 2.28
M059K mCherry:EGFP + drug 2.88, 2.59 2.77, 2.25
M059J mCherry:EGFP 2.97, 3.75 1.71, 2.40
M059K mCherry:damaged EGFP 0.11, 0.12 0.10, 0.14
M059K mCherry:damaged EGFP + drug 0.09, 0.09 0.07, 0.06
M059J mCherry:damaged EGFP 0.04, 0.02 0.02, 0.01


WF Purple Team: PstI-BglII, DMNB

Sample Normalized EGFP/mCherry value (replicate #1) Normalized EGFP/mCherry value (replicate #2)
M059K mCherry:EGFP 2.42, 2.43 2.21, 2.58
M059K mCherry:EGFP + drug 2.21, 2.20 2.38, 2.79
M059J mCherry:EGFP 3.20, 2.99 2.59, 2.20
M059K mCherry:damaged EGFP 0.18, 0.15 0.18, 0.18
M059K mCherry:damaged EGFP + drug 0.19, 0.61 0.03, 0.03
M059J mCherry:damaged EGFP 0, 0 0, 0
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