Difference between revisions of "Talk:20.109(S16):Design mutation primers (Day2)"
From Course Wiki
Liz Strand (Talk | contribs) (→Primers for SDM) |
|||
| Line 40: | Line 40: | ||
|5' TTTTGACAAGcATGGGAACGGC 3' | |5' TTTTGACAAGcATGGGAACGGC 3' | ||
|5' ACACGGAATGCTTCTCGG 3' | |5' ACACGGAATGCTTCTCGG 3' | ||
| − | | | + | | D94H |
|- | |- | ||
|<font color = #FF0000>WF red</font color> | |<font color = #FF0000>WF red</font color> | ||
Revision as of 14:01, 10 February 2016
Primers for SDM
| Team | Forward primer | Reverse primer | Mutation (X#Z in CaM) |
| TR red | 5' CACCACAAAGagACTTGGCACCGTTATG 3' | 5' ATGGTGCCGTCCCCATCC 3' | |
| TR orange | 5' ATTCGACAAGcatGGGGACGGCA 3' | 5' AATGAGAAGGCTTCTTTGAACTCTG 3' | |
| TR yellow | CACCACAAAGaAACTTGGCACCG | ATGGTGCCGTCCCCATCC | |
| TR green | 5' insert here 3' | 5' insert here 3' | |
| TR blue | 5' insert here 3' | 5' insert here 3' | |
| TR pink | 5' GTT GAT GAA ATG ATA AGG GAA cCA GAT ATC GAT GGT GAT GGC C 3' | 5' TTC TTC ATC TGT TAA CTT CTC CCC GAG GTT TGT CAT 3' | |
| TR purple | 5' TTTTGACAAGcATGGGAACGGC 3' | 5' ACACGGAATGCTTCTCGG 3' | D94H |
| WF red | 5' insert here 3' | 5' insert here 3' | |
| WF orange | 5' insert here 3' | 5' insert here 3' | |
| WF blue | 5' insert here 3' | 5' insert here 3' | |
| WF pink | 5' insert here 3' | 5' insert here 3' | |
| WF purple | 5' insert here 3' | 5' insert here 3' |
