Difference between revisions of "Talk:20.109(F17):Design gRNA for CRISPRi (Day2)"
From Course Wiki
(→Fermentation product and gene targeted:) |
(→W/F) |
||
| Line 74: | Line 74: | ||
|- | |- | ||
|blue | |blue | ||
| − | | | + | | Ethanol |
| − | | | + | | ackA |
| − | | | + | | TTTTTAGCCACGTATCAATTAT |
|} | |} | ||
Revision as of 18:29, 18 October 2017
Fermentation product and gene targeted:
T/R
| Team | Ethanol (E) or Acetate (A) | Gene targeted by CRISPRi gRNA | gRNA sequence (without tag at 3' end) |
| red | Acetate | adhE | CTTAACGCACTCGTAGAGCGTGTAAA |
| orange | Ethanol | pta-ack | CTATGGCTCCCTGACGTTTT |
| yellow | Ethanol | frdA | GCTGTGGGATAAAAACAATCTGGAG |
| green | E | LdhA | ttgtgctataaacggcgagtttcat |
| blue | E | pta | ttcacgacaacgttcaataatcat |
| pink | Acetate | adhE | TTACTAAAAAAGTTTAACATTATCA |
| purple | Acetate | adhE | GATTTACTAAAAAAGTTTAACATTATC |
| white | Acetate | adhE | CTGATAATGTTAAACTTTTT |
W/F
| Team | Ethanol (E) or Acetate (A) | Gene targeted by CRISPRi gRNA | gRNA sequence (without tag at 3' end) |
| red | |||
| orange | |||
| green | |||
| blue | Ethanol | ackA | TTTTTAGCCACGTATCAATTAT |
