Difference between revisions of "Talk:20.109(F16):Module 2"
From Course Wiki
Sami Kebede (Talk | contribs) (→T/R: lactate) |
(→T/R: lactate) |
||
| Line 16: | Line 16: | ||
|pflB | |pflB | ||
|TGTCGAAGTACGCAGTAAAT | |TGTCGAAGTACGCAGTAAAT | ||
| − | | | + | |putative promoter |
|- | |- | ||
|green | |green | ||
Revision as of 18:57, 3 November 2016
Contents
Module 2 Data
M2D2: gRNA design
T/R: lactate
| Team | Gene targeted by CRISPRi gRNA |
gRNA sequence (without tag at 3' end) |
Locus targeted (e.g. beginning of gene, putative promoter, -35 region) |
| yellow | pflB | TGTCGAAGTACGCAGTAAAT | putative promoter |
| green | pflB | ATAAAAAATCCACTTAAGAAGGTA | |
| blue | pflB | TTCATTAAGCTCGGACATGTAACA | End of Putative Promoter & beginning of pflB gene |
| pink | pflB | AAATAAAAAATCCACTTAAGAAGGT | Putative Promoter |
| purple | pflB | AAATCCACTTAAGAAGGTAGGTGT |
W/F: ethanol
| Team | Gene targeted by CRISPRi gRNA |
gRNA sequence (without tag at 3' end) |
Locus targeted (e.g. beginning of gene, putative promoter, -35 region) |
| red | pta-ack | GCC ACG TAT CAA TTA TAG GTA C | |
| green | ldhA | GTAGCTTAAATGTGATTCAACATC | |
| blue | fdrA | CAAGATCGGCTTGAAAGGTTTGCAC | |
| purple | ldhA | TCGTACTGTTTTGTGCTATAAA |
Note: The dCas9 recognition sequence is 5' - GTTTTAGAGCTAGAAATAGCAAGTTAAAATAAGGC - 3'.
M2D5/M2D7: sequencing data
T/R
| Team | pgRNA sequencing |
| yellow | files |
| green | files |
| blue | files |
| pink | files |
| purple | files |
W/F
| Team | pgRNA sequencing |
| red | files |
| green | files |
| blue | files |
| purple | files |
