Talk:20.109(F16):Design gRNA for CRISPRi (Day2)

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Fermentation product and gene targeted:

T/R

Team Ethanol (E) or lactate (L) Gene targeted by CRISPRi gRNA gRNA sequence (without tag at 3' end)
yellow L pflB TGTCGAAGTACGCAGTAAAT
green L pflB ATAAAAAATCCACTTAAGAAGGTA
blue L pflB TTCATTAAGCTCGGACATGTAACA
pink L pflB AAATAAAAAATCCACTTAAGAAGGT
purple L pflB AAATCCACTTAAGAAGGTAGGTGT white L pflB AAATAAAAAATCCACTTAAGAAGGT

W/F

Team Ethanol (E) or lactate (L) Gene targeted by CRISPRi gRNA gRNA sequence (without tag at 3' end)
red E pta-ack GCC ACG TAT CAA TTA TAG GTA C
green E ldhA GTAGCTTAAATGTGATTCAACATC
blue E fdrA CAAGATCGGCTTGAAAGGTTTGCAC
purple E ldhA TCGTACTGTTTTGTGCTATAAA