Difference between revisions of "Talk:20.109(F16):Design gRNA for CRISPRi (Day2)"

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(Fermentation product and gene targeted:)
(T/R)
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|pflB
 
|pflB
 
|AAATCCACTTAAGAAGGTAGGTGT
 
|AAATCCACTTAAGAAGGTAGGTGT
 +
|white
 +
|L
 +
|pflB
 +
|AAATAAAAAATCCACTTAAGAAGGT
 +
|-
 
|}
 
|}
  

Revision as of 18:40, 17 October 2017

Fermentation product and gene targeted:

T/R

Team Ethanol (E) or lactate (L) Gene targeted by CRISPRi gRNA gRNA sequence (without tag at 3' end)
yellow L pflB TGTCGAAGTACGCAGTAAAT
green L pflB ATAAAAAATCCACTTAAGAAGGTA
blue L pflB TTCATTAAGCTCGGACATGTAACA
pink L pflB AAATAAAAAATCCACTTAAGAAGGT
purple L pflB AAATCCACTTAAGAAGGTAGGTGT white L pflB AAATAAAAAATCCACTTAAGAAGGT

W/F

Team Ethanol (E) or lactate (L) Gene targeted by CRISPRi gRNA gRNA sequence (without tag at 3' end)
red E pta-ack GCC ACG TAT CAA TTA TAG GTA C
green E ldhA GTAGCTTAAATGTGATTCAACATC
blue E fdrA CAAGATCGGCTTGAAAGGTTTGCAC
purple E ldhA TCGTACTGTTTTGTGCTATAAA