Difference between revisions of "Talk:20.109(F16):Design gRNA for CRISPRi (Day2)"

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(Fermentation product and gene targeted:)
(T/R)
Line 12: Line 12:
 
|L
 
|L
 
|pflB
 
|pflB
|GCAGTAAATAAAAAATCCACTTAAG
+
|TGTCGAAGTACGCAGTAAAT
 
|-
 
|-
 
|green
 
|green

Revision as of 19:50, 18 October 2016

Fermentation product and gene targeted:

T/R

Team Ethanol (E) or lactate (L) Gene targeted by CRISPRi gRNA gRNA sequence (without tag at 3' end)
yellow L pflB TGTCGAAGTACGCAGTAAAT
green L pflB ATAAAAAATCCACTTAAGAAGGTA
blue
pink L pflB AAATAAAAAATCCACTTAAGAAGGT
purple L pflB AAATCCACTTAAGAAGGTAGGTGT

W/F

Team Ethanol (E) or lactate (L) Gene targeted by CRISPRi gRNA gRNA sequence (without tag at 3' end)
red
green
blue
purple