Difference between revisions of "Talk:20.109(F16):Design gRNA for CRISPRi (Day2)"

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|L
 
|L
 
|pflB
 
|pflB
−
|
+
|TGTCGAAGTACGCAGTAAAT
 
|-
 
|-
 
|green
 
|green
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|-
 
|-
 
|blue
 
|blue
−
|
+
|E
−
|
+
|pta
−
|
+
|ttcacgacaacgttcaataatcat
 
|-
 
|-
 
|pink
 
|pink
 
|L
 
|L
 
|pflB
 
|pflB
−
|
+
|AAATAAAAAATCCACTTAAGAAGGT
 
|-
 
|-
 
|purple
 
|purple
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|pflB
 
|pflB
 
|AAATCCACTTAAGAAGGTAGGTGT
 
|AAATCCACTTAAGAAGGTAGGTGT
 +
|white
 +
|L
 +
|pflB
 +
|AAATAAAAAATCCACTTAAGAAGGT
 +
|-
 
|}
 
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|-
 
|-
 
|red
 
|red
−
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+
|E
−
|
+
|pta-ack
−
|
+
|GCC ACG TAT CAA TTA TAG GTA C
 
|-
 
|-
 
|green
 
|green
−
|
+
|E
−
|
+
|ldhA
−
|
+
|GTAGCTTAAATGTGATTCAACATC
 
|-
 
|-
 
|blue
 
|blue
−
|
+
|E
−
|
+
|fdrA
−
|
+
|CAAGATCGGCTTGAAAGGTTTGCAC
 
|-
 
|-
 
|purple
 
|purple
−
|
+
|E
−
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|ldhA
−
|
+
|TCGTACTGTTTTGTGCTATAAA
 
|}
 
|}

Latest revision as of 19:08, 17 October 2017

Fermentation product and gene targeted:

T/R

Team Ethanol (E) or lactate (L) Gene targeted by CRISPRi gRNA gRNA sequence (without tag at 3' end)
yellow L pflB TGTCGAAGTACGCAGTAAAT
green L pflB ATAAAAAATCCACTTAAGAAGGTA
blue E pta ttcacgacaacgttcaataatcat
pink L pflB AAATAAAAAATCCACTTAAGAAGGT
purple L pflB AAATCCACTTAAGAAGGTAGGTGT white L pflB AAATAAAAAATCCACTTAAGAAGGT

W/F

Team Ethanol (E) or lactate (L) Gene targeted by CRISPRi gRNA gRNA sequence (without tag at 3' end)
red E pta-ack GCC ACG TAT CAA TTA TAG GTA C
green E ldhA GTAGCTTAAATGTGATTCAACATC
blue E fdrA CAAGATCGGCTTGAAAGGTTTGCAC
purple E ldhA TCGTACTGTTTTGTGCTATAAA
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