Difference between revisions of "Talk:20.109(F16):Design gRNA for CRISPRi (Day2)"
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MAXINE JONAS (Talk | contribs) (→T/R) |
(→Fermentation product and gene targeted:) |
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|'''Gene targeted by CRISPRi gRNA''' | |'''Gene targeted by CRISPRi gRNA''' | ||
|'''gRNA sequence (without tag at 3' end)''' | |'''gRNA sequence (without tag at 3' end)''' | ||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
|- | |- | ||
|yellow | |yellow | ||
| − | | | + | |L |
| − | | | + | |pflB |
| − | | | + | |TGTCGAAGTACGCAGTAAAT |
|- | |- | ||
|green | |green | ||
| − | | | + | |L |
| − | | | + | |pflB |
| − | | | + | |ATAAAAAATCCACTTAAGAAGGTA |
|- | |- | ||
|blue | |blue | ||
| − | | | + | |E |
| − | | | + | |pta |
| − | | | + | |ttcacgacaacgttcaataatcat |
| + | |- | ||
| + | |pink | ||
| + | |L | ||
| + | |pflB | ||
| + | |AAATAAAAAATCCACTTAAGAAGGT | ||
| + | |- | ||
| + | |purple | ||
| + | |L | ||
| + | |pflB | ||
| + | |AAATCCACTTAAGAAGGTAGGTGT | ||
| + | |white | ||
| + | |L | ||
| + | |pflB | ||
| + | |AAATAAAAAATCCACTTAAGAAGGT | ||
| + | |- | ||
|} | |} | ||
| Line 41: | Line 46: | ||
|'''Ethanol (E) or lactate (L)''' | |'''Ethanol (E) or lactate (L)''' | ||
|'''Gene targeted by CRISPRi gRNA''' | |'''Gene targeted by CRISPRi gRNA''' | ||
| + | |'''gRNA sequence (without tag at 3' end)''' | ||
|- | |- | ||
|red | |red | ||
| − | | | + | |E |
| − | | | + | |pta-ack |
| − | + | |GCC ACG TAT CAA TTA TAG GTA C | |
| − | | | + | |
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
|- | |- | ||
|green | |green | ||
| − | | | + | |E |
| − | | | + | |ldhA |
| + | |GTAGCTTAAATGTGATTCAACATC | ||
|- | |- | ||
|blue | |blue | ||
| − | | | + | |E |
| − | | | + | |fdrA |
| + | |CAAGATCGGCTTGAAAGGTTTGCAC | ||
| + | |- | ||
| + | |purple | ||
| + | |E | ||
| + | |ldhA | ||
| + | |TCGTACTGTTTTGTGCTATAAA | ||
|} | |} | ||
Latest revision as of 19:08, 17 October 2017
Fermentation product and gene targeted:
T/R
| Team | Ethanol (E) or lactate (L) | Gene targeted by CRISPRi gRNA | gRNA sequence (without tag at 3' end) | ||||
| yellow | L | pflB | TGTCGAAGTACGCAGTAAAT | ||||
| green | L | pflB | ATAAAAAATCCACTTAAGAAGGTA | ||||
| blue | E | pta | ttcacgacaacgttcaataatcat | ||||
| pink | L | pflB | AAATAAAAAATCCACTTAAGAAGGT | ||||
| purple | L | pflB | AAATCCACTTAAGAAGGTAGGTGT | white | L | pflB | AAATAAAAAATCCACTTAAGAAGGT |
W/F
| Team | Ethanol (E) or lactate (L) | Gene targeted by CRISPRi gRNA | gRNA sequence (without tag at 3' end) |
| red | E | pta-ack | GCC ACG TAT CAA TTA TAG GTA C |
| green | E | ldhA | GTAGCTTAAATGTGATTCAACATC |
| blue | E | fdrA | CAAGATCGGCTTGAAAGGTTTGCAC |
| purple | E | ldhA | TCGTACTGTTTTGTGCTATAAA |
