Difference between revisions of "Talk:20.109(F16):Design gRNA for CRISPRi (Day2)"

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(Fermentation product and gene targeted:)
(T/R)
Line 16: Line 16:
 
|green
 
|green
 
|L
 
|L
|pfl
+
|pflB
|
+
|ATAAAAAATCCACTTAAGAAGGTA
 
|-
 
|-
 
|blue
 
|blue

Revision as of 19:38, 18 October 2016

Fermentation product and gene targeted:

T/R

Team Ethanol (E) or lactate (L) Gene targeted by CRISPRi gRNA gRNA sequence (without tag at 3' end)
yellow L pflB
green L pflB ATAAAAAATCCACTTAAGAAGGTA
blue
pink L pflB
purple L pflB AAATCCACTTAAGAAGGTAGGTGT

W/F

Team Ethanol (E) or lactate (L) Gene targeted by CRISPRi gRNA gRNA sequence (without tag at 3' end)
red
green
blue
purple