Talk:20.109(F15):Evaluate mutations and site-directed mutagenesis (Day1)
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Revision as of 20:48, 8 October 2015 by Christie Contreras (Talk | contribs)
IPC mutants (and primers) chosen by teams
T/R RED
- mutation: D132H
- forward primer:
- reverse primer:
- restriction enzyme:
T/R ORANGE
- mutation: T78P
- forward primer:
- reverse primer:
- restriction enzyme:
T/R GREEN
- mutation: D57H
- forward primer:
- reverse primer:
- restriction enzyme:
T/R BLUE
- mutation: D132H
- forward primer:
- reverse primer:
- restriction enzyme:
T/R PINK
- mutation: D132H
- forward primer: 5' GCC GGC TAT CGA TGG TCA TGG CCA AGT AAA C 3'
- reverse primer:
- restriction enzyme: NaeI (GCC / GGC)
