Difference between revisions of "Talk:20.109(F15):Evaluate mutations and site-directed mutagenesis (Day1)"
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(→IPC mutants (and primers) chosen by teams) |
(→IPC mutants (and primers) chosen by teams) |
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*restriction enzyme: | *restriction enzyme: | ||
<br><font color = #FF0000>'''W/F RED'''</font color> | <br><font color = #FF0000>'''W/F RED'''</font color> | ||
| − | *mutation: | + | *mutation: |
| − | *forward primer: | + | *forward primer: |
| − | *reverse primer: | + | *reverse primer: |
| − | *restriction enzyme: | + | *restriction enzyme: |
<br><font color = #FF6600>'''T/R ORANGE'''</font color> | <br><font color = #FF6600>'''T/R ORANGE'''</font color> | ||
*mutation: T78P | *mutation: T78P | ||
| Line 17: | Line 17: | ||
<br><font color = #FFFF00>'''W/F YELLOW'''</font color> | <br><font color = #FFFF00>'''W/F YELLOW'''</font color> | ||
*mutation: D21A | *mutation: D21A | ||
| − | *forward primer: | + | *forward primer: 5' GCC GGC ATT CGA CAA GGC TGG GGA CGG CA |
| − | *reverse primer: | + | *reverse primer: 5' AAT GAG AAG GCT TCT TTG AAC TCT GC |
| − | *restriction enzyme: | + | *restriction enzyme: NaeI - GCCGGC |
<br><font color = #00FF00>'''T/R GREEN'''</font color> | <br><font color = #00FF00>'''T/R GREEN'''</font color> | ||
*mutation: D57H | *mutation: D57H | ||
Revision as of 19:26, 9 October 2015
IPC mutants (and primers) chosen by teams
T/R RED
- mutation: D132H
- forward primer:
- reverse primer:
- restriction enzyme:
W/F RED
- mutation:
- forward primer:
- reverse primer:
- restriction enzyme:
T/R ORANGE
- mutation: T78P
- forward primer:
- reverse primer:
- restriction enzyme:
W/F YELLOW
- mutation: D21A
- forward primer: 5' GCC GGC ATT CGA CAA GGC TGG GGA CGG CA
- reverse primer: 5' AAT GAG AAG GCT TCT TTG AAC TCT GC
- restriction enzyme: NaeI - GCCGGC
T/R GREEN
- mutation: D57H
- forward primer:
- reverse primer:
- restriction enzyme:
W/F GREEN
- mutation:
- forward primer:
- reverse primer:
- restriction enzyme:
T/R BLUE
- mutation: D132H
- forward primer:
- reverse primer:
- restriction enzyme:
W/F BLUE
- mutation:
- forward primer:
- reverse primer:
- restriction enzyme:
T/R PINK
- mutation: D132H
- forward primer: 5' GCC GGC TAT CGA TGG TCA TGG CCA AGT AAA C 3'
- reverse primer: 5'TCT GCT TCC CTT ATC ATT TC 3'
- restriction enzyme: NaeI (GCC / GGC)
W/F PINK
- mutation:
- forward primer:
- reverse primer:
- restriction enzyme:
