Difference between revisions of "Talk:20.109(F15):Evaluate mutations and site-directed mutagenesis (Day1)"
From Course Wiki
(→IPC mutants (and primers) chosen by teams) |
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*mutation: D132H | *mutation: D132H | ||
*forward primer: 5' GCC GGC TAT CGA TGG TCA TGG CCA AGT AAA C 3' | *forward primer: 5' GCC GGC TAT CGA TGG TCA TGG CCA AGT AAA C 3' | ||
| − | *reverse primer: | + | *reverse primer: 5'TCT GCT TCC CTT ATC ATT TC 3' |
*restriction enzyme: NaeI (GCC / GGC) | *restriction enzyme: NaeI (GCC / GGC) | ||
Revision as of 21:23, 8 October 2015
IPC mutants (and primers) chosen by teams
T/R RED
- mutation: D132H
- forward primer:
- reverse primer:
- restriction enzyme:
T/R ORANGE
- mutation: T78P
- forward primer:
- reverse primer:
- restriction enzyme:
T/R GREEN
- mutation: D57H
- forward primer:
- reverse primer:
- restriction enzyme:
T/R BLUE
- mutation: D132H
- forward primer:
- reverse primer:
- restriction enzyme:
T/R PINK
- mutation: D132H
- forward primer: 5' GCC GGC TAT CGA TGG TCA TGG CCA AGT AAA C 3'
- reverse primer: 5'TCT GCT TCC CTT ATC ATT TC 3'
- restriction enzyme: NaeI (GCC / GGC)
