Difference between revisions of "Talk:20.109(F15):Evaluate mutations and site-directed mutagenesis (Day1)"
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<br><font color = #FF66CC>'''T/R PINK'''</font color> | <br><font color = #FF66CC>'''T/R PINK'''</font color> | ||
*mutation: D132H | *mutation: D132H | ||
| − | *forward primer: | + | *forward primer: 5' GCC GGC TAT CGA TGG TCA TGG CCA AGT AAA C 3' |
*reverse primer: | *reverse primer: | ||
| − | *restriction enzyme: | + | *restriction enzyme: NaeI (GCC / GGC) |
Revision as of 20:48, 8 October 2015
IPC mutants (and primers) chosen by teams
T/R RED
- mutation: D132H
- forward primer:
- reverse primer:
- restriction enzyme:
T/R ORANGE
- mutation: T78P
- forward primer:
- reverse primer:
- restriction enzyme:
T/R GREEN
- mutation: D57H
- forward primer:
- reverse primer:
- restriction enzyme:
T/R BLUE
- mutation: D132H
- forward primer:
- reverse primer:
- restriction enzyme:
T/R PINK
- mutation: D132H
- forward primer: 5' GCC GGC TAT CGA TGG TCA TGG CCA AGT AAA C 3'
- reverse primer:
- restriction enzyme: NaeI (GCC / GGC)
