Difference between revisions of "Spring 2012:Vincent Lee Lab 2"
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== Objectives == | == Objectives == | ||
− | * | + | * Building a fluorescence microscope, tuning |
+ | * Learning how optical components like those from ThorLabs come together and work. | ||
+ | * Learning about stepper control in a lab setting | ||
+ | * Learning about 3-dimensional deconvolution and looking at the differences in image reconstruction for the actual and theoretical PSF. | ||
+ | |||
+ | |||
+ | == Materials == | ||
+ | === Microscope === | ||
+ | * Epi-fluorescence Microscope<ref>[[Lab_Manual:Optical_Microscopy]]</ref> with parts from Thorlabs | ||
+ | === Drivers === | ||
+ | [[File:A4988Diagram.png|200px|thumb|<b>Figure 1.</b> The Pololu A4983 Microstepper with voltage regulators.]] | ||
+ | * Pololu A4983 Stepper Motor Driver Carrier with Voltage Regulators<ref name="A4983">[http://www.pololu.com/catalog/product/1202 A4983 Stepper Motor Driver Carrier with Voltage Regulators]</ref> | ||
+ | * Thorlabs 8 mm Travel Stepper Motor Actuator<ref>[http://www.thorlabs.com/newgrouppage9.cfm?objectgroup_id=1881 8 mm Travel Stepper Motor Actuator]</ref> | ||
+ | === Processing === | ||
+ | * Matlab | ||
+ | * Matlab Image Acquisition Toolbox<ref>[http://www.mathworks.com/products/imaq/ Mathworks Image Acquisition Toolbox]</ref> | ||
+ | * Matlab Allied Vision Technologies Adaptor<ref>[http://www.alliedvisiontec.com/emea/products/software/3rd-party-solutions/mathworks.html Matlab Allied Vision Technologies Adaptor]</ref> | ||
+ | * National Instruments USB Data Acquisition<ref>[http://sine.ni.com/nips/cds/view/p/lang/en/nid/203223 National Instruments USB Data Acquisition 6210]</ref> | ||
+ | * ImageJ<ref>[http://rsbweb.nih.gov/ij/ ImageJ - Image Processing and Analysis in Java]</ref> | ||
+ | * ImageJ plugins: Deconvolution Lab<ref>[http://bigwww.epfl.ch/algorithms/deconvolutionlab/ ImageJ Plugin - DeconvolutionLab]</ref> and Diffraction PSF 3D<ref>[http://www.optinav.com/Diffraction-PSF-3D.htm ImageJ Plugin - Diffraction PSF 3D]</ref>. Manually save in the plugins folder in ImageJ. | ||
+ | === Subject === | ||
+ | * Submicron beads, small enough to be considered point sources | ||
+ | |||
+ | == Microscope == | ||
+ | > | ||
+ | === Safety === | ||
+ | > | ||
+ | === Alignment === |
Revision as of 15:10, 8 May 2012
Contents
Objectives
- Building a fluorescence microscope, tuning
- Learning how optical components like those from ThorLabs come together and work.
- Learning about stepper control in a lab setting
- Learning about 3-dimensional deconvolution and looking at the differences in image reconstruction for the actual and theoretical PSF.
Materials
Microscope
- Epi-fluorescence Microscope[1] with parts from Thorlabs
Drivers
- Pololu A4983 Stepper Motor Driver Carrier with Voltage Regulators[2]
- Thorlabs 8 mm Travel Stepper Motor Actuator[3]
Processing
- Matlab
- Matlab Image Acquisition Toolbox[4]
- Matlab Allied Vision Technologies Adaptor[5]
- National Instruments USB Data Acquisition[6]
- ImageJ[7]
- ImageJ plugins: Deconvolution Lab[8] and Diffraction PSF 3D[9]. Manually save in the plugins folder in ImageJ.
Subject
- Submicron beads, small enough to be considered point sources
Microscope
>
Safety
>
Alignment
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