Spring 2011:3D PSF lab
From Course Wiki
Revision as of 17:57, 11 February 2011 by Gustavo Goretkin (Talk | contribs)
Objectives
- Measure the point spread function of the 20.309 fluorescence microscope
- Examine the effect of NA, magnification, and optical corrections on PSF
- Generate a dataset for deconvolution problem set
Materials and methods
Exercise left to the reader
Microscope at Station 5
The microscope currently at station 5 is not finalized. Bright field images reveal the sample along with a ghost image of the sample due to some reflection. One possible reason is that the 200mm imaging lens is too far from the objective.
