Spring 2011:3D PSF lab

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20.309: Biological Instrumentation and Measurement

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Objectives

  • Measure the point spread function of the 20.309 fluorescence microscope
  • Examine the effect of NA, magnification, and optical corrections on PSF
  • Generate a dataset for deconvolution problem set

Materials and methods

Exercise left to the reader

Links

Pololu Stepper Motor Driver

ThorLabs DRV001 Stepper Motor Drive

ThorLabs NanoMax Stages