Weekly Progress

Weekend/Monday

Initial chambers for testing cells & fluorescent beads

Finalized parts list.
Continued coding image analysis / segmentation code.
Fabricated Microfluidics using resources from Kamm lab and Voldemann lab.
- PDMS poured & cured Monday afternoon
Moved microscope setup to larger breadboard.

Will start a culture of I13521 from freezer stock. ~12 hours of grow time should be sufficient. Also, potentially growing up KAF95 and miniprepping it may prove useful if we can BioBrick out the fliC273 gene.

David Liu has agreed to give us MP-QUR, pickup will be TBD, but we can hopefully just go over to Harvard and get the plasmid.
The Elowitz Repressilator can apparently be found on Add-Gene, will need to either find or figure out a good testing rig to put it in.
Cory has I13521 and I13522, although both are in pSB1A3, which might prove problematic if the KAF95 fliC is also on a pBR322 derived plasmid. Current backup is J04450 which can be found in pSB1C3 and would allow for a co-transformant.