Revision as of 06:06, 19 April 2011

Weekly Progress

Initial chambers for testing cells & fluorescent beads

Finalized parts list.
Continued coding image analysis / segmentation code.
Fabricated Microfluidics using resources from Kamm lab and Voldemann lab.
- PDMS poured & cured Monday afternoon
Moved microscope setup to larger breadboard.

2 Cultures of I13521 started from freezer stocks tubes 2&3. Aliquoted into 5mL of LB+Amp and left to grow overnight. ~12 hours of growth time should be sufficient for experimentation tomorrow.
Idea: potentially growing up KAF95 and miniprepping it may prove useful if we can BioBrick out the fliC273 gene.

David Liu has agreed to give us MP-QUR, pickup will be TBD, but we can hopefully just go over to Harvard and get the plasmid.
The Elowitz Repressilator can apparently be found on Add-Gene, will need to either find or figure out a good testing rig to put it in.
Cory has I13521 and I13522, although both are in pSB1A3, which might prove problematic if the KAF95 fliC is also on a pBR322 derived plasmid. Current backup is J04450 which can be found in pSB1C3 and would allow for a co-transformant.

@@ Line 9: / Line 9: @@
 ==Monday Evening==
-* Will start a culture of I13521 from freezer stock.  ~12 hours of grow time should be sufficient.  Also, potentially growing up KAF95 and miniprepping it may prove useful if we can BioBrick out the ''fliC273'' gene.
+* 2 Cultures of I13521 started from freezer stocks tubes 2&3.  Aliquoted into 5mL of LB+Amp and left to grow overnight.  ~12 hours of growth time should be sufficient for experimentation tomorrow.
+* Idea: potentially growing up KAF95 and miniprepping it may prove useful if we can BioBrick out the ''fliC273'' gene.
 =Notes=