Spring 11:Chemotaxis Assay

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Microinjector Seymour.jpg


Introduction

For my project I designed and developed an automated microfluidic system using Seymour et al’s microinjector microfluidic device to provide a high through-put method of acquiring bacterial chemotaxis parameters. The microinjector device was chosen for its simple design, diverse application to both fluid-flow and flow-free approaches, and for its ability to measure individual cell kinetics. The development of a faster and systematic device is especially useful in the research of marine microbes’ response to settling dissolved organic matter.

I improved the

Objective

Further develop Stocker’s microfluidic system that will allow researchers to run chemotaxis assays more efficiently for high though-put assays. This objective will be achieved by

Spring 20.345 Project Assignments

Readings and Resources

Microinjector Microfluidic Device

Pneumatic System Design

Basic Design

Circuit Element Design

Experiment 1:

weekly reports


- By Emmanuel Quiroz