Difference between revisions of "Spring 11:Chemotaxis Assay"
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== Introduction == | == Introduction == | ||
For my project I designed and developed an automated microfluidic system using Seymour et al’s microinjector microfluidic device to provide a high through-put method of acquiring bacterial chemotaxis parameters. The microinjector device was chosen for its simple design, diverse application to both fluid-flow and flow-free approaches, and for its ability to measure individual cell kinetics. The development of a faster and systematic device is especially useful in the research of marine microbes’ response to settling dissolved organic matter. | For my project I designed and developed an automated microfluidic system using Seymour et al’s microinjector microfluidic device to provide a high through-put method of acquiring bacterial chemotaxis parameters. The microinjector device was chosen for its simple design, diverse application to both fluid-flow and flow-free approaches, and for its ability to measure individual cell kinetics. The development of a faster and systematic device is especially useful in the research of marine microbes’ response to settling dissolved organic matter. | ||
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== Objective == | == Objective == | ||
| − | Further develop Stocker’s microfluidic system that will allow researchers to run chemotaxis assays more efficiently for high though-put assays. | + | Further develop Stocker’s microfluidic system that will allow researchers to run chemotaxis assays more efficiently for high though-put assays. The main improvement to Stocker's system will be the use of pneumatic pressures instead of syringe fluid pressures to control fluid flow to set up the chemoatractant and microorganism bands. The pneumatic pressure will be computer controlled to to allow precise and reproducible experiments as well as increased experimental throughput. |
| − | + | ||
=== Spring 20.345 Project Assignments === | === Spring 20.345 Project Assignments === | ||
| + | * [add files here, ask steve] | ||
== Readings and Resources == | == Readings and Resources == | ||
| + | * [add files and links here, ask steve] | ||
== Microinjector Microfluidic Device == | == Microinjector Microfluidic Device == | ||
Revision as of 20:33, 16 May 2011
Contents
Introduction
For my project I designed and developed an automated microfluidic system using Seymour et al’s microinjector microfluidic device to provide a high through-put method of acquiring bacterial chemotaxis parameters. The microinjector device was chosen for its simple design, diverse application to both fluid-flow and flow-free approaches, and for its ability to measure individual cell kinetics. The development of a faster and systematic device is especially useful in the research of marine microbes’ response to settling dissolved organic matter.
Objective
Further develop Stocker’s microfluidic system that will allow researchers to run chemotaxis assays more efficiently for high though-put assays. The main improvement to Stocker's system will be the use of pneumatic pressures instead of syringe fluid pressures to control fluid flow to set up the chemoatractant and microorganism bands. The pneumatic pressure will be computer controlled to to allow precise and reproducible experiments as well as increased experimental throughput.
Spring 20.345 Project Assignments
- [add files here, ask steve]
Readings and Resources
- [add files and links here, ask steve]
Microinjector Microfluidic Device
Pneumatic System Design
Basic Design
Circuit Element Design
Experiment 1:
- By Emmanuel Quiroz
