Difference between revisions of "Spring 11:Chemotaxis Assay"
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== Introduction == | == Introduction == | ||
| + | For my project I designed and developed an automated microfluidic system using Seymour et al’s microinjector microfluidic device to provide a high through-put method of acquiring bacterial chemotaxis parameters. The microinjector device was chosen for its simple design, diverse application to both fluid-flow and flow-free approaches, and for its ability to measure individual cell kinetics. The development of a faster and systematic device is especially useful in the research of marine microbes’ response to settling dissolved organic matter. | ||
| + | I improved the | ||
| + | |||
| + | == Objective == | ||
| + | Further develop Stocker’s microfluidic system that will allow researchers to run chemotaxis assays more efficiently for high though-put assays. This objective will be achieved by | ||
=== Spring 20.345 Project Assignments === | === Spring 20.345 Project Assignments === | ||
| + | |||
| + | == Readings and Resources == | ||
| + | |||
| + | == Microinjector Microfluidic Device == | ||
| + | |||
| + | == Pneumatic System Design == | ||
| + | |||
| + | === Basic Design === | ||
| + | |||
| + | === Circuit Element Design === | ||
| + | == Experiment 1: == | ||
[[Spring_11 ChemotaxisAssayWeeklyReports|weekly reports]] | [[Spring_11 ChemotaxisAssayWeeklyReports|weekly reports]] | ||
Revision as of 19:57, 16 May 2011
Contents
Introduction
For my project I designed and developed an automated microfluidic system using Seymour et al’s microinjector microfluidic device to provide a high through-put method of acquiring bacterial chemotaxis parameters. The microinjector device was chosen for its simple design, diverse application to both fluid-flow and flow-free approaches, and for its ability to measure individual cell kinetics. The development of a faster and systematic device is especially useful in the research of marine microbes’ response to settling dissolved organic matter.
I improved the
Objective
Further develop Stocker’s microfluidic system that will allow researchers to run chemotaxis assays more efficiently for high though-put assays. This objective will be achieved by
Spring 20.345 Project Assignments
Readings and Resources
Microinjector Microfluidic Device
Pneumatic System Design
Basic Design
Circuit Element Design
Experiment 1:
- By Emmanuel Quiroz
