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• 20.109(F14): Mod 1 Day 6 Lipofection
  ...rize yourself with these mechanisms by (re)reading the excellent review by Thomas Helleday that you can find in the References section of the [[20.109(F14):M ...ive cells with low temperature agarose (this means it melts around 37 °C) and casting a small gel on a microscope slide. Cells were exposed to DNA d
  14 KB (2,341 words) - 14:02, 29 July 2015
• 20.109(F13):Module 1
  ...ll also test whether this N-terminally truncated EGFP can recombine with a C-terminally truncated version to regenerate full length EGFP in vivo. Finall ...er treatment'''<br>''DNA Repair'' 2007<br> Thomas Helleday, Justin Lo, Dik C. van Gent, Bevin P. Engelward<br> [http://dx.doi.org/10.1016/j.dnarep.2007.
  5 KB (652 words) - 17:33, 29 July 2015
• 20.109(F13): Mod 1 Day 1 DNA engineering using PCR
  ...rmation on homologous recombination, please obtain the excellent review by Thomas Helleday from the References section of the [[20.109(F13):Module 1| Module ...ll be detecting recombination between an N-terminally truncated EGFP and a C-terminally truncated version. The primers you are designing today will be u
  21 KB (3,687 words) - 17:33, 29 July 2015
• 20.109(F13): Mod 1 Day 6 Lipofection and paper discussion
  ...rize yourself with these mechanisms by (re)reading the excellent review by Thomas Helleday that you can find in the References section of the [[20.109(F13):M *7. Return the plate to the 37&deg;C incubator.
  12 KB (1,875 words) - 17:33, 29 July 2015
• 20.109(F13):Journal club II (Day8)
  ...pecific ovarian cancer research.'' Anglesio MS, Wiegand KC, Melnyk N, Chow C, et al. '''PLoS One.''' 2013 Sep 4;8(9):e72162. [http://openwetware.org/wik ... by comparison of genomic profiles.'' Domcke S, Sinha R, Levine DA, Sander C, Schultz N. '''Nat Commun.''' 2013;4:2126. [http://openwetware.org/wiki/DOI
  16 KB (2,323 words) - 17:33, 29 July 2015
• 20.109(F12):Module 1
  ...ll also test whether this N-terminally truncated EGFP can recombine with a C-terminally truncated version to regenerate full length EGFP in vivo. Finall ...er treatment'''<br>''DNA Repair'' 2007<br> Thomas Helleday, Justin Lo, Dik C. van Gent, Bevin P. Engelward<br> [http://web.mit.edu/engelward-lab/publica
  4 KB (561 words) - 13:41, 29 July 2015
• 20.109(F12): Mod 1 Day 1 DNA engineering using PCR
  ...rmation on homologous recombination, please obtain the excellent review by Thomas Helleday from the References section of the [[20.109(F12):Module 1| Module ...ll be detecting recombination between an N-terminally truncated EGFP and a C-terminally truncated version. The primers you are designing today will be u
  22 KB (3,714 words) - 13:41, 29 July 2015
• 20.109(F12): Mod 1 Day 6 Lipofection & lab practical
  ...rize yourself with these mechanisms by (re)reading the excellent review by Thomas Helleday that you can find in the References section of the [[20.109(F12):M ...different things we might test, and we will use wells labeled 'A' 'B' and 'C' to test a parameter that might affect the frequency of inter-plasmid homol
  9 KB (1,495 words) - 13:41, 29 July 2015
• 20.109(F15):Module 1
  ...ll also test whether this N-terminally truncated EGFP can recombine with a C-terminally truncated version to regenerate full length EGFP ''in vivo''. Fi ...er treatment'''<br>''DNA Repair'' 2007<br> Thomas Helleday, Justin Lo, Dik C. van Gent, Bevin P. Engelward<br> [http://dx.doi.org/10.1016/j.dnarep.2007.
  4 KB (602 words) - 13:32, 10 September 2015
• 20.109(F15):DNA engineering using PCR (Day1)
  ...formation on homologous recombination, please read the excellent review by Thomas Helleday from the References section of the [[20.109(F15):Module 1| Module ...ll be detecting recombination between an N-terminally truncated EGFP and a C-terminally truncated version. The primers you are designing today will be u
  21 KB (3,638 words) - 13:47, 2 October 2015
• 20.109(F15):Lipofection (Day6)
  ...rize yourself with these mechanisms by (re)reading the excellent review by Thomas Helleday that you can find in the References section of the [[20.109(F15):M ...ive cells with low temperature agarose (this means it melts around 37 &deg;C) and casting a small gel on a microscope slide. Cells were exposed to DNA d
  16 KB (2,572 words) - 17:58, 1 October 2015
• 20.109(F16):DNA engineering using PCR (Day1)
  ...formation on homologous recombination, please read the excellent review by Thomas Helleday from the References section of the [[20.109(F16):Module 1| Module ...ll be detecting recombination between an N-terminally truncated EGFP and a C-terminally truncated version. The primers you are designing today will be u
  21 KB (3,638 words) - 14:58, 18 May 2016
• 20.109(F16):Lipofection (Day6)
  ...rize yourself with these mechanisms by (re)reading the excellent review by Thomas Helleday that you can find in the References section of the [[20.109(F16):M ...ive cells with low temperature agarose (this means it melts around 37 &deg;C) and casting a small gel on a microscope slide. Cells were exposed to DNA d
  16 KB (2,572 words) - 15:33, 18 May 2016