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20.109(S15):Diagnostic primer analysis (Day8)20.109(S15):Diagnostic primer design (Day2)20.109(S15):Flow cytometry and paper discussion (Day6)
20.109(S15):General lab policy20.109(S15):Growth of phage nanowires (Day 1)20.109(S15):Guidelines for maintaining your lab notebook
20.109(S15):Guidelines for oral presentations20.109(S15):Guidelines for working in the tissue culture facility20.109(S15):Guidelines for writing up your research
20.109(S15):Introduction to cell strains and plating (Day1)20.109(S15):Journal club II (Day9)20.109(S15):Journal club I (Day6)
20.109(S15):Lab basics20.109(S15):Lab tour20.109(S15):Microbial DNA extraction (Day1)
20.109(S15):Microbiome data summary20.109(S15):Module 120.109(S15):Module 2
20.109(S15):Module 320.109(S15):NHEJ system research article20.109(S15):OWW basics
20.109(S15):People20.109(S15):Phylogenetic analysis (Day7)20.109(S15):Phylogentic analysis (Day8)
20.109(S15):Primer design memo20.109(S15):Research proposal presentation20.109(S15):Solar cell assembly (Day 4)
20.109(S15):Solar cell testing (Day 5)20.109(S15):Statement on collaboration and integrity20.109(S15):Student proposal presentations (Day7)
20.109(S15):Student registration and questionnaire20.109(S15):Wrap-up and data summary (Day 6)20.109(S15): Assignments
20.109(S15): Biomaterial engineering report20.109(S15): Complete Western and prepare damaged DNA (Day3)20.109(S15): Mod 3 Day 1 Growth of phage materials
20.109(S15): Mod 3 Day 1 Growth of phage materials (Day 1)20.109(S15): Spring 2015 schedule20.109(S15): TA notes for module 1
20.109(S15): TA notes for module 220.109(S15): TEM (Day 3)20.109(S16)
20.109(S16):20.109 Class Blog summary page20.109(S16):Assess protein function (Day8)20.109(S16):Battery assembly and testing (Day5)
20.109(S16):Begin Western blot protein analysis and choose system conditions (Day2)20.109(S16):Cathode construction (Day3)20.109(S16):Cell preparation for DNA repair assays (Day5)
20.109(S16):Characterize protein expression (Day7)20.109(S16):Complete Western and prepare damaged DNA (Day3)20.109(S16):DNA repair assays (Day6)
20.109(S16):Data analysis (Day9)20.109(S16):Design mutation primers (Day2)20.109(S16):Flow cytometry and paper discussion (Day7)
20.109(S16):General lab policy20.109(S16):Growth of phage materials (Day1)20.109(S16):Guidelines for maintaining your lab notebook
20.109(S16):Guidelines for oral presentations20.109(S16):Guidelines for working in the tissue culture facility20.109(S16):Guidelines for writing up your research
20.109(S16):Homework20.109(S16):In situ cloning (Day1)20.109(S16):Induce protein expression (Day5)
20.109(S16):Introduction to cell strains and plating (Day1)20.109(S16):Journal Club II (Day8)20.109(S16):Journal Club I (Day4)
20.109(S16):Lab basics20.109(S16):Lab tour20.109(S16):Module 1
20.109(S16):Module 220.109(S16):Module 320.109(S16):OWW basics
20.109(S16):People20.109(S16):Prepare expression system (Day4)20.109(S16):Purify active materials (Day2)
20.109(S16):Purify protein (Day6)20.109(S16):Research proposal presentation20.109(S16):Research proposal presentations (Day6)
20.109(S16):Site-directed mutagenesis (Day3)20.109(S16):Statement on collaboration and integrity20.109(S16):Student registration and questionnaire
20.109(S16):TEM (Day4)20.109(S16): Assignments20.109(S16): Biomaterials engineering report
20.109(S16): Protein engineering presentation20.109(S16): Protein engineering summary20.109(S16): Spring 2016 schedule
20.109(S16): System engineering report20.109(S16): TA notes for module 120.109(S16): TA notes for module 2
20.109(S16): TA notes for module 320.109(S16): TA notes for orientation20.109(S17):Analyze RNA-seq data (Day7)
20.109(S17):Assess cell survival and harvest RNA for quantitative PCR assay (Day3)20.109(S17):Battery assembly and testing (Day5)20.109(S17):Cathode construction (Day3)
20.109(S17):Communication20.109(S17):Complete Western blot and induce DNA damage for survival and quantitative PCR assays (Day2)20.109(S17):Complete cell survival assay and examine transcript levels in response to DNA damage (Day 4)
20.109(S17):Complete cell viability assay (Day9)20.109(S17):Complete data analysis (Day6)20.109(S17):Confirm cell lines and practice tissue culture techniques (Day1)
20.109(S17):Evaluation of purified protein (Day3)20.109(S17):Examine quantitative PCR results (Day9)20.109(S17):Examine transcript levels in response to DNA damage (Day 4)
20.109(S17):Growth of phage materials (Day1)20.109(S17):Guidelines for working in the tissue culture facility20.109(S17):Homework
20.109(S17):Identify chemical structures common among FKBP12 binders20.109(S17):In silico cloning and induction of protein expression (Day1)20.109(S17):Journal Club II (Day8)
20.109(S17):Journal Club I (Day5)20.109(S17):Lab tour20.109(S17):Module 1
20.109(S17):Module 220.109(S17):Module 320.109(S17):Practice data analysis methods (Day6)
20.109(S17):Prepare Western blot and induce DNA damage for quantitative PCR assay (Day2)20.109(S17):Purification of induced protein (Day2)20.109(S17):Purify RNA for quantitative PCR assay and treat cells for survival assay (Day3)
20.109(S17):Purify active materials (Day2)20.109(S17):Research proposal presentation20.109(S17):Scan slides to identify FKBP12 binders (Day5)
20.109(S17):Screen chemical library for FKBP12 binders (Day4)20.109(S17):TEM (Day4)20.109(S17): Announcements
20.109(S17): Assignments20.109(S17): Biomaterials engineering mini-report20.109(S17): Fall 2016 schedule
20.109(S17): M1 Data Summary20.109(S17): M1 Mini-presentation20.109(S17): Research article
20.109(S17): Spring 2017 schedule20.109(S17): TA notes for M320.109(S17): TA notes for orientation
20.109(S18):Analyze RNA-seq data and prepare for quantitative PCR experiment (Day 4)20.109(S18):Analyze small microarray data (Day3)20.109(S18):Analyze small microarray data and induce protein expression (Day2)
20.109(S18):Assemble and test batteries (Day5)20.109(S18):Class data20.109(S18):Communication
20.109(S18):Complete data analysis (Day7)20.109(S18):Confirm ligand binding using differential scanning fluorimetry assay (Day6)20.109(S18):Construct cathode with phage-based active material (Day3)
20.109(S18):Evaluate protein purity and concentration (Day4)20.109(S18):Growth of phage-based active (cathode) material (Day1)20.109(S18):Homework
20.109(S18):In silico cloning and confirmation digest of protein expression vector(Day1)20.109(S18):Induce DNA damage and apply drug treatments for RNA purification (Day2)20.109(S18):Induce DNA damage and apply drug treatments for cell viability and identification of regulatory motifs in RNA-seq data (Day8)
20.109(S18):Investigate RNA-seq data using public databases (Day5)20.109(S18):Journal club presentations (Day6 and 7)20.109(S18):Laboratory tour
20.109(S18):Module 120.109(S18):Module 220.109(S18):Module 3
20.109(S18):Practice tissue culture techniques and prepare cells for RNA purification (Day1)20.109(S18):Prep notes for M320.109(S18):Purify RNA and practice RNA-seq data analysis methods (Day3)
20.109(S18):Purify phage-based active (cathode) material (Day2)20.109(S18):Purify protein for secondary assays(Day3)20.109(S18):Research proposal presentation
20.109(S18):SECONDARY ASSAY D2 (Day6)20.109(S18):Test protein activity using peptidyl-prolyl cis-trans isomerase assay (Day5)20.109(S18):Visualize phage nanowires using TEM (Day4)
20.109(S18): Announcements20.109(S18): Assignments20.109(S18): Biomaterials engineering mini-report
20.109(S18): M1 Data Summary20.109(S18): M1 Mini-presentation20.109(S18): Prep notes for M1
20.109(S18): Prep notes for M220.109(S18): Research article20.109(S18): Spring 2018 schedule
20.109(S18): TA notes for orientation20.109(S19):Analyze RNA-seq data and prepare for quantitative PCR experiment (Day 4)20.109(S19):Assemble and test batteries (Day5)
20.109(S19):Class data20.109(S19):Communication20.109(S19):Complete data analysis (Day7)
20.109(S19):Complete data analysis (Day9)20.109(S19):Confirm ligand binding using differential scanning fluorimetry assay (Day6)20.109(S19):Construct cathode with phage-based active material (Day3)
20.109(S19):Design cell viability experiment and complete RNA-seq data analysis (Day6)20.109(S19):Etoposide treat cells for RNA purification (Day2)20.109(S19):Evaluate protein purity and concentration (Day4)
20.109(S19):Growth of phage-based active (cathode) material (Day1)20.109(S19):Guidelines for working in the tissue culture facility20.109(S19):Homework
20.109(S19):In silico cloning and confirmation digest of protein expression vector(Day1)20.109(S19):Journal club presentations (Day7 and 8)20.109(S19):Laboratory tour
20.109(S19):Module 120.109(S19):Module 220.109(S19):Module 3
20.109(S19):Perform quantitative PCR experiment and explore additional RNA-seq datasets (Day5)20.109(S19):Practice tissue culture techniques and prepare cells for RNA purification (Day1)20.109(S19):Prep notes for M3
20.109(S19):Purify RNA and practice RNA-seq data analysis methods (Day3)20.109(S19):Purify phage-based active (cathode) material (Day2)20.109(S19):Purify protein for secondary assays(Day2)
20.109(S19):Research proposal presentation20.109(S19):Test protein activity using peptidyl-prolyl cis-trans isomerase assay (Day5)20.109(S19):Visualize phage nanowires using TEM (Day4)
20.109(S19): Announcements20.109(S19): Assignments20.109(S19): Data Summary
20.109(S19): M1 Mini-presentation20.109(S19): Mini-presentation20.109(S19): Mini-report
20.109(S19): Prep notes, orientation20.109(S19): Prep notes for M120.109(S19): Prep notes for M2
20.109(S19): Research article20.109(S19): Spring 2019 schedule20.109(S20):Analyze RNA-seq data and select gene targets for quantitative PCR (qPCR) experiment (Day 6)
20.109(S20):Analyze SMM data (Day6)20.109(S20):Analyze titration curves (Day5)20.109(S20):Analyze titration curves (Day6)
20.109(S20):Assess purity and concentration of TDP43 protein (Day3)20.109(S20):Characterize clone by titration using flow cytometry (Day4)20.109(S20):Characterize clone by titration using flow cytometry (Day5)
20.109(S20):Class data20.109(S20):Communication20.109(S20):Complete in silico cloning and induce TDP43 protein expression (Day1)
20.109(S20):Enrich candidate clones using fluorescence-activated cell sorter (FACS) (Day1)20.109(S20):Examine TDP43 binders for chemical features (Day7)20.109(S20):Examine qPCR results and complete data analysis (Day8)
20.109(S20):Guidelines for working in the tissue culture facility20.109(S20):Harvest candidate clones (Day2)20.109(S20):Homework
20.109(S20):Identify clone to characterize (Day4)20.109(S20):Identify clones to characterize (Day3)20.109(S20):Journal club presentation (Day4 and 6)
20.109(S20):Journal club presentations (Day4 and 5)20.109(S20):Laboratory tour20.109(S20):Module 1
20.109(S20):Module 220.109(S20):Module 320.109(S20):Perform qPCR experiment and continue RNA-seq data analysis (Day7)
20.109(S20):Perform small molecule microarray (SMM) with TDP43 protein (Day4)20.109(S20):Prepare cells for RNA purification and practice RNA-seq data analysis methods (Day1)20.109(S20):Prepare clones for sequencing (Day3)
20.109(S20):Purify RNA from etoposide-treated cells and and generate cDNA (Day3)20.109(S20):Purify TDP43 protein (Day2)20.109(S20):Research proposal presentation
20.109(S20):Scan SMM slides to identify binders of TDP43 protein (Day5)20.109(S20):Treat cells with etoposide and analyze RNA-seq data (Day2)20.109(S20): Announcements
20.109(S20): Assignments20.109(S20): Data Summary20.109(S20): Mini-presentation
20.109(S20): Mini-report20.109(S20): Prep notes, orientation20.109(S20): Prep notes for M1
20.109(S20): Research article20.109(S20): Spring 2020 schedule20.109(S21):Announcements
20.109(S21):Assignments20.109(S21):Communication20.109(S21):Data Summary
20.109(S21):Guidelines for working in the tissue culture facility20.109(S21):Homework20.109(S21):Journal club presentation
20.109(S21):Laboratory tour20.109(S21):M1D120.109(S21):M1D2
20.109(S21):M1D320.109(S21):M1D420.109(S21):M1D5
20.109(S21):M1D620.109(S21):M1D720.109(S21):M2D1
20.109(S21):M2D220.109(S21):M2D320.109(S21):M2D4
20.109(S21):M2D520.109(S21):M2D620.109(S21):M2D7
20.109(S21):M3D120.109(S21):M3D220.109(S21):M3D3
20.109(S21):M3D420.109(S21):M3D520.109(S21):Mini-presentation
20.109(S21):Mini-report20.109(S21):Module 120.109(S21):Module 2
20.109(S21):Module 320.109(S21):Research article20.109(S21):Research proposal presentation
20.109(S21):Spring 2021 schedule20.109(S22):Assignments20.109(S22):Class data
20.109(S22):Communication20.109(S22):Data Summary20.109(S22):FYI
20.109(S22):Homework20.109(S22):Journal club presentation20.109(S22):Laboratory tour
20.109(S22):M1D120.109(S22):M1D220.109(S22):M1D3
20.109(S22):M1D420.109(S22):M1D520.109(S22):M1D6
20.109(S22):M1D720.109(S22):M1D820.109(S22):M2D1
20.109(S22):M2D220.109(S22):M2D320.109(S22):M2D4
20.109(S22):M2D520.109(S22):M2D620.109(S22):M2D7
20.109(S22):M2D820.109(S22):M3D120.109(S22):M3D2
20.109(S22):M3D320.109(S22):M3D420.109(S22):Module 1
20.109(S22):Module 220.109(S22):Module 320.109(S22):Research article
20.109(S22):Research proposal presentation20.109(S22):Research talk20.109(S22):Spring 2022 schedule
20.109:Lab basics20.109:Module 120.109:Module 2
20.109:Module 320.109:Module 420.109:OWW basics
20.109:People20.109: Spring 2007 schedule20.109 (F07): Phage by design
20.109 (S07): Atomic force microscopy20.109 (S07): Transmission electron microscopy20.109 Main Page
20.309:Course Information20.309:DAQ System20.309:Lab Report Guidelines
20.309:Learning outcomes20.309:Safety20.309: Exam 1 Topics