Difference between revisions of "Results"
(Created page with "== Bead experiments == These experiments were performed with the 532 nm setup, and the 100 nm PSF beads prepared in the lab before. 4 different data sets were captured, with dif...") |
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These experiments were performed with the 532 nm setup, and the 100 nm PSF beads prepared in the lab before. 4 different data sets were captured, with different fields of view and exposure times of 10, 30, 60 and 100 ms. These data sets were analyzed using the QuickPALM software as an exercise to better learn the technique. | These experiments were performed with the 532 nm setup, and the 100 nm PSF beads prepared in the lab before. 4 different data sets were captured, with different fields of view and exposure times of 10, 30, 60 and 100 ms. These data sets were analyzed using the QuickPALM software as an exercise to better learn the technique. | ||
| − | Aside from the default parameters used for all 4 data sets, we sampled the parameter space with ranges of 3 parameters in the image processing, namely, the minimum signal to noise ratio accepted by the algorithm, the maximum full width at half maximum tolerated, and the expected symmetry of the particles. | + | Aside from the default parameters used for all 4 data sets, we sampled the parameter space with ranges of 3 parameters in the image processing, namely, the minimum signal to noise ratio accepted by the algorithm (values of 4, 8, 12, and 16), the maximum full width at half maximum tolerated (values of 2, 4, 8 and 20 nm), and the expected symmetry of the particles (values of 0, 50 and 90%). Additionally, the SpheroTech beads used for all the previous experiments were compared at different exposure times with the Invitrogen PS-Speck deep red beads using different optics and laser. |
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| + | If not stated at the bottom right corner of each image, the parameters used for the experiment were SNR of 12, FWHM of 4 nm and Symmetry of 50%. | ||
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| + | The figures are located at a [http://web.mit.edu/~dguggian/public public folder] | ||
== Cell experiments == | == Cell experiments == | ||
Revision as of 19:08, 19 May 2011
Bead experiments
These experiments were performed with the 532 nm setup, and the 100 nm PSF beads prepared in the lab before. 4 different data sets were captured, with different fields of view and exposure times of 10, 30, 60 and 100 ms. These data sets were analyzed using the QuickPALM software as an exercise to better learn the technique.
Aside from the default parameters used for all 4 data sets, we sampled the parameter space with ranges of 3 parameters in the image processing, namely, the minimum signal to noise ratio accepted by the algorithm (values of 4, 8, 12, and 16), the maximum full width at half maximum tolerated (values of 2, 4, 8 and 20 nm), and the expected symmetry of the particles (values of 0, 50 and 90%). Additionally, the SpheroTech beads used for all the previous experiments were compared at different exposure times with the Invitrogen PS-Speck deep red beads using different optics and laser.
If not stated at the bottom right corner of each image, the parameters used for the experiment were SNR of 12, FWHM of 4 nm and Symmetry of 50%.
The figures are located at a public folder
