Difference between revisions of "Resource list: DNA melting and PCR"
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| + | ==DNA melting thermodynamics== | ||
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| + | * Ryan T. Koehler and Nicolas Peyret [http://bioinformatics.oxfordjournals.org/cgi/content/full/21/16/3333 Thermodynamic properties of DNA sequences: characteristic values for the human genome] Bioinformatics 2005 21(16):3333-3339; doi:10.1093/bioinformatics/bti530 | ||
==Use of fluorescent reportes to measure DNA melting curves== | ==Use of fluorescent reportes to measure DNA melting curves== | ||
Revision as of 20:22, 6 June 2010
DNA melting thermodynamics
- Ryan T. Koehler and Nicolas Peyret Thermodynamic properties of DNA sequences: characteristic values for the human genome Bioinformatics 2005 21(16):3333-3339; doi:10.1093/bioinformatics/bti530
Use of fluorescent reportes to measure DNA melting curves
- Ririe, et al. Product Differentiation by Analysis of DNA Melting Curves during the Polymerase Chain Reaction. Analytical Biochemistry 245, 154–160 (1997)
- Michael T. Bjorndal and D. Kuchnir Fygenson. DNA Melting in the Presence of Fluorescent Intercalating Oxazole Yellow Dyes Measured with a Gel-Based Assay. Biopolymers, Vol. 65, 40–44 (2002)
Applications of DNA melting curves
- Worm, et. al. In-Tube DNA Methylation Profiling by Fluorescence Melting Curve Analysis Clinical Chemistry 47:7 1183–1189 (2001)
- Marziliano, et. al. Melting Temperature Assay for a UGT1A Gene Variant in Gilbert Syndrome. Clinical Chemistry. 2000;46:423-425.) (Also see: Ahsen, et. al. Limitations of Genotyping Based on Amplicon Melting Temperature. Clinical Chemistry. 2001;47:1331-1332.
Real-time PCR
- Kubista, et. al. The real-time polymerase chain reaction Molecular Aspects of Medicine 27 (2006) 95–125.
