Photobleaching Model

Trying to figure out a better model for the photobleaching of Sybr Green I in the DNA melting lab, because our current model fails at long times.

What is the current model?

The Matlab DNA melting simulation writeup has a fixed percentage of the active fluorophores being destroyed every time step. The bleaching coefficient B is set such that half the fluorophores will have been destroyed by the end of the simulation, if you had a constant input.

I think this makes at least some sense, because if you have the fluorescence be low and then go up at the end, then the bleached is more than half of the unbleached, because there were inactive fluorophores not being destroyed... argh I am confused.

How is it done in EstimateDnaMeltingParameters?

How does Sybr Green actually photobleach?

Photobleaching is "a very poorly understood phenomenon" [1]

To a first approximation, every fluorescence event has an equal chance of causing the destruction of that one fluorophor.