Difference between revisions of "Photobleaching Model"
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Photobleaching is "a very poorly understood phenomenon" [http://micro.magnet.fsu.edu/primer/java/fluorescence/photobleaching/] | Photobleaching is "a very poorly understood phenomenon" [http://micro.magnet.fsu.edu/primer/java/fluorescence/photobleaching/] | ||
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| + | To a first approximation, every fluorescence event has an equal chance of causing the destruction of that one fluorophor. | ||
Revision as of 19:23, 7 July 2011
Trying to figure out a better model for the photobleaching of Sybr Green I in the DNA melting lab, because our current model fails at long times.
What is the current model?
The Matlab DNA melting simulation writeup has a fixed percentage of the fluorophores being destroyed every time step. VERIFY THIS
How does Sybr Green actually photobleach?
Photobleaching is "a very poorly understood phenomenon" [1]
To a first approximation, every fluorescence event has an equal chance of causing the destruction of that one fluorophor.
