Difference between revisions of "Optical Microscopy: Part 4 Report Outline"

From Course Wiki
Jump to: navigation, search
Line 8: Line 8:
 
         <ul>
 
         <ul>
 
           <li>Include a snapshot of the 0.84 &mu;m fluorescent beads monitored.</li>
 
           <li>Include a snapshot of the 0.84 &mu;m fluorescent beads monitored.</li>
           <li>Plot one or more example bead trajectories for each of the samples. </li>
+
           <li>Plot two or more example bead trajectories for each of the samples. (Hint: If you subtract the initial position from each trajectory, then you can plot multiple trajectories on a single set of axes.)</li>
 
         </ul>
 
         </ul>
 
       <li>Analysis and Results</li>
 
       <li>Analysis and Results</li>
 
         <ul>
 
         <ul>
 +
          <li>Plot the average MSD vs τ results for all glycerin samples (A, B, C, and D); use log-log axes. Use the minimum number of axes that can convey your results clearly.</li>
 
           <li>Include a table of the diffusion coefficient, viscosity and glycerin/water ratio for each of the samples (A, B, C, and D).</li>
 
           <li>Include a table of the diffusion coefficient, viscosity and glycerin/water ratio for each of the samples (A, B, C, and D).</li>
 
           <li>Plot the average MSD for all three PVP samples on a single set of log-log axes (0h, 1h, and 3h).</li>
 
           <li>Plot the average MSD for all three PVP samples on a single set of log-log axes (0h, 1h, and 3h).</li>

Revision as of 21:51, 20 October 2015

  1. Viscosity
    1. Procedure
      • Document the samples you prepared and used and how you captured images (camera settings including frame acquisition rate, number of frames, number of particles in the region of interest, choice of sample plane, etc)
    2. Data
      • Include a snapshot of the 0.84 μm fluorescent beads monitored.
      • Plot two or more example bead trajectories for each of the samples. (Hint: If you subtract the initial position from each trajectory, then you can plot multiple trajectories on a single set of axes.)
    3. Analysis and Results
      • Plot the average MSD vs τ results for all glycerin samples (A, B, C, and D); use log-log axes. Use the minimum number of axes that can convey your results clearly.
      • Include a table of the diffusion coefficient, viscosity and glycerin/water ratio for each of the samples (A, B, C, and D).
      • Plot the average MSD for all three PVP samples on a single set of log-log axes (0h, 1h, and 3h).
      • Provide a bullet point outline of all calculations and data processing steps.
    4. Discussion
      • How do your viscosity calculations compare to your expectations? (This chart is a useful reference.)
      • What can you infer about the viscoelastic properties of the PVP gels from your MSD plots?
      • Include a thorough discussion of error sources and the approaches to minimize them.