Difference between revisions of "Optical Microscopy: Part 4 Report Outline"

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           <li>What can you infer about the viscoelastic properties of the PVP gels from your MSD plots?</li>
 
           <li>What can you infer about the viscoelastic properties of the PVP gels from your MSD plots?</li>
 
           <li>Include a thorough discussion of error sources and the approaches to minimize them.</li>
 
           <li>Include a thorough discussion of error sources and the approaches to minimize them.</li>
 +
        </ul>
 +
    </ol>
 +
  </li>
 +
  </li> 
 +
</ol>
 +
<ol start="6">
 +
  </li>  <li>Particle Tracking Microrheology in Cells
 +
    <ol>
 +
      <li>Procedure
 +
        <ul><li>Document the samples you prepared and used and how you captured images (camera settings including frame acquisition rate, number of frames, number of particles in the region of interest, choice of sample plane, etc)</li></ul>
 +
      </li>
 +
      <li>Data</li>
 +
        <ul>
 +
          <li>Include a snapshot of the 0.84 &mu;m fluorescent beads monitored.</li>
 +
          <li>Plot two or more example bead trajectories for each of the samples. (Hint: If you subtract the initial position from each trajectory, then you can plot multiple trajectories on a single set of axes.)</li>
 +
        </ul>
 +
      <li>Analysis and Results</li>
 +
        <ul>
 +
          <li>Plot the average MSD vs τ results for untreated and Cyto D treated cells.</li>
 +
          <li>Plot the average MSD for untreated and treated cells on a single set of log-log axes.</li>
 +
        </ul>
 +
      <li>Discussion</li>
 +
        <ul>
 +
          <li>What kind of motion do you see described by your MSD vs τ results?</li>
 +
          <li>What differences do you see between the untreated and Cyto D treated MSD curves? </li>
 +
          <li>Please suggest an interpretation of the microrheological behavior of your cells based on your data.</li>
 
         </ul>
 
         </ul>
 
     </ol>
 
     </ol>

Revision as of 20:05, 24 February 2016

  1. Viscosity
    1. Procedure
      • Document the samples you prepared and used and how you captured images (camera settings including frame acquisition rate, number of frames, number of particles in the region of interest, choice of sample plane, etc)
    2. Data
      • Include a snapshot of the 0.84 μm fluorescent beads monitored.
      • Plot two or more example bead trajectories for each of the samples. (Hint: If you subtract the initial position from each trajectory, then you can plot multiple trajectories on a single set of axes.)
    3. Analysis and Results
      • Plot the average MSD vs τ results for all glycerin samples (A, B, C, and D); use log-log axes. Use the minimum number of axes that can convey your results clearly.
      • Include a table of the diffusion coefficient, viscosity and glycerin/water ratio for each of the samples (A, B, C, and D).
      • Plot the average MSD for all three PVP samples on a single set of log-log axes (0h, 1h, and 3h).
      • Provide a bullet point outline of all calculations and data processing steps.
    4. Discussion
      • How do your viscosity calculations compare to your expectations? (This chart is a useful reference.)
      • What can you infer about the viscoelastic properties of the PVP gels from your MSD plots?
      • Include a thorough discussion of error sources and the approaches to minimize them.
  1. Particle Tracking Microrheology in Cells
    1. Procedure
      • Document the samples you prepared and used and how you captured images (camera settings including frame acquisition rate, number of frames, number of particles in the region of interest, choice of sample plane, etc)
    2. Data
      • Include a snapshot of the 0.84 μm fluorescent beads monitored.
      • Plot two or more example bead trajectories for each of the samples. (Hint: If you subtract the initial position from each trajectory, then you can plot multiple trajectories on a single set of axes.)
    3. Analysis and Results
      • Plot the average MSD vs τ results for untreated and Cyto D treated cells.
      • Plot the average MSD for untreated and treated cells on a single set of log-log axes.
    4. Discussion
      • What kind of motion do you see described by your MSD vs τ results?
      • What differences do you see between the untreated and Cyto D treated MSD curves?
      • Please suggest an interpretation of the microrheological behavior of your cells based on your data.
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