Optical Microscopy: Part 3 Report Outline

5. Disrupting and imaging the actin network
   1. Procedure
      • Document sample preparation, including difficulties, and recording conditions (particularly exposure time and gain selected in the camera settings)
   2. Data
   • Include images of mouse embryonic fibroblasts + and - CytoD.
   3. Analysis and Results
   • What contrast do you unveil between the + and - CytoD conditions?
   4. Discussion
   • Can you quantify the morphological and physiological effect of CytoD treatment? What parameters would you choose to measure and report?
6. Resolution
   1. Procedure
      • Document the samples you used and how you captured images (camera settings, software used, etc…)
   2. Data
   • Include an image of the PSF sample with the beads used for resolution measurement indicated.
   3. Analysis and Results
   • Report the resolution you measured. Make sure to include N and a measure of uncertainty.
   • Show sample Gaussian fits.
   • Explain the Matlab algorithm used for data analysis.
   4. Discussion
   • Compare the measured value to the theoretical value.
   • Include a thorough discussion of error sources.
7. Stability
   1. Procedure
      • Document the samples you used and how you captured images (camera settings, frame rate, total number of frames, exposure, software used, etc…)
   2. Data
   • Show an example frame from the stability movie.
   • Provide X-Y plots of difference tracks for pairs of fixed particles.
   • Plot MSD versus time interval τ for sum and difference tracks. Use a linear or logarithmic vertical axis so as to most clearly illustrate the relationship between the two datasets.
   3. Analysis and Results
   • Provide a bullet point outline of data analysis methodology.
   • Include a thorough discussion of error sources.
   4. Discussion
   • What are the benefits and drawbacks of differential tracking?