Difference between revisions of "Optical Microscopy: Part 3 Report Outline"

Revision as of 17:40, 14 October 2014

5. Disrupting and imaging the actin network
   1. Procedure
      • Document sample preparation, including difficulties, and recording conditions (particularly exposure time and gain selected in the camera settings)
   2. Data
   • Include images of mouse embryonic fibroblasts + and - CytoD.
   3. Analysis and Results
   • What contrast do you unveil between the + and - CytoD conditions?
   4. Discussion
   • Can you quantify the morphological and physiological effect of CytoD treatment? What parameters would you choose to measure and report?
6. Resolution
   1. Procedure
      • Document the samples you used and how you captured images (camera settings, software used, etc…)
   2. Data
   • Include an image of the PSF sample with the beads used for resolution measurement indicated.
   3. Analysis and Results
   • Report the resolution you measured. Make sure to include N and a measure of uncertainty.
   • Show sample Gaussian fits.
   • Explain the Matlab algorithm used for data analysis.
   4. Discussion
   • Compare the measured value to the theoretical value.
   • Include a thorough discussion of error sources. Do not comment on insignificant sources of error. To determine which error sources are significant, and which are not, you must think carefully about the uncertainty related to each error source and estimate its magnitude and sign. Include these estimates in your report along with your estimate of the combined, total uncertainty.
7. Stability
   1. Procedure
      • Document the samples you used and how you captured images (camera settings, frame rate, total number of frames, exposure, software used, etc…)
   2. Data
   • Show an example frame from the stability movie.
   • Provide X-Y plots of difference tracks for pairs of fixed particles.
   • Plot MSD versus time interval τ for individual and difference tracks. Use a linear or logarithmic vertical axis so as to most clearly illustrate the relationship between the two datasets.
   3. Analysis and Results
   • Provide a bullet point outline of data analysis methodology.
   • Include a thorough discussion of error sources.
   4. Discussion
   • What are the benefits and drawbacks of differential tracking?