Difference between revisions of "Optical Microscopy: Part 3 Report Outline"

From Course Wiki
Jump to: navigation, search
m
Line 1: Line 1:
 
<ol start="5">
 
<ol start="5">
 +
</li>  <li>Disrupting and imaging the actin network
 +
    <ol>
 +
      <li>Procedure
 +
        <ul><li>Document sample preparation, including difficulties, and recording conditions (particularly exposure time and gain selected in the camera settings)</li></ul>
 +
      </li>
 +
      <li>Data</li>
 +
        <ul>
 +
          <li>Include images of mouse embryonic fibroblasts + and - CytoD.</li>
 +
        </ul>
 +
      <li>Analysis and Results</li>
 +
        <ul>
 +
          <li>What contrast do you unveil between the + and - CytoD conditions?</li>
 +
        </ul>
 +
      <li>Discussion</li>
 +
        <ul>
 +
          <li>Can you quantify the morphological and physiological effect of CytoD treatment?  What parameters would you choose to measure and report?</li>
 +
        </ul>
 +
    </ol>
 +
  </li>
 +
  </li> 
 +
 
   </li>  <li>Resolution
 
   </li>  <li>Resolution
 
     <ol>
 
     <ol>

Revision as of 19:22, 17 September 2014

  1. Disrupting and imaging the actin network
    1. Procedure
      • Document sample preparation, including difficulties, and recording conditions (particularly exposure time and gain selected in the camera settings)
    2. Data
      • Include images of mouse embryonic fibroblasts + and - CytoD.
    3. Analysis and Results
      • What contrast do you unveil between the + and - CytoD conditions?
    4. Discussion
      • Can you quantify the morphological and physiological effect of CytoD treatment? What parameters would you choose to measure and report?
  2. Resolution
    1. Procedure
      • Document the samples you used and how you captured images (camera settings, software used, etc…)
    2. Data
      • Include an image of the PSF sample with the beads used for resolution measurement indicated.
    3. Analysis and Results
      • Report the resolution you measured. Make sure to include N and a measure of uncertainty.
      • Show sample Gaussian fits.
      • Explain the Matlab algorithm used for data analysis.
    4. Discussion
      • Compare the measured value to the theoretical value.
      • Include a thorough discussion of error sources.
  3. Stability
    1. Procedure
      • Document the samples you used and how you captured images (camera settings, frame rate, total number of frames, exposure, software used, etc…)
    2. Data
      • Show an example frame from the stability movie.
      • Provide X-Y plots of difference tracks for pairs of fixed particles.
      • Plot MSD versus time interval τ for sum and difference tracks. Use a linear or logarithmic vertical axis so as to most clearly illustrate the relationship between the two datasets.
    3. Analysis and Results
      • Provide a bullet point outline of data analysis methodology.
      • Include a thorough discussion of error sources.
    4. Discussion
      • What are the benefits and drawbacks of differential tracking?
Retrieved from "http://measurebiology.org/w/index.php?title=Optical_Microscopy:_Part_3_Report_Outline&oldid=9726"