View source for Optical Microscopy: Part 3 Report Outline


<ol start="6">
 
  </li>  <li>Resolution
    <ol>
      <li>Procedure
        <ul><li>Document the samples you used and how you captured images (camera settings, software used, etc…)</li></ul>
      </li>
      <li>Data</li>
        <ul>
          <li>Include an image of the PSF sample indicating which beads were used for resolution measurement..</li>
        </ul>
      <li>Analysis and Results</li>
        <ul>
          <li>Report the resolution you measured. Make sure to include N and a measure of uncertainty.</li>
          <li>Show sample Gaussian fits.</li>
          <li>Explain the Matlab algorithm used for data analysis.</li>
        </ul>
      <li>Discussion</li>
        <ul>
          <li>Compare the measured value to the theoretical value.</li>
          <li>Include a thorough discussion of error sources. Do not comment on insignificant sources of error. To determine which error sources are significant, and which are not, you must think carefully about the uncertainty related to each error source and estimate its magnitude and sign. Include these estimates in your report along with your estimate of the combined, total uncertainty. It may be helpful to list out the error sources in a table, including a category for the error source, type of error (random, systematic, fundamental, technical, etc.), the magnitude of the error, and a description and way to minimize each one.</li>
        </ul>
    </ol>
  </li>
  </li>  
  <li>Stability
    <ol>
      <li>Procedure
        <ul><li>Document the samples you used and how you captured images (camera settings, frame rate, total number of frames, exposure, software used, etc…)</li></ul>
      </li>
      <li>Data</li>
        <ul>
          <li>Show an example frame from the stability movie.</li>
          <li>Plot two or more example bead trajectories for each of the samples. (Hint: If you subtract the initial position from each trajectory, then you can plot multiple trajectories on a single set of axes.)
        </ul>
      <li>Analysis and Results</li>
        <ul>
          <li>Plot MSD versus time interval &tau; for individual and difference tracks using log-log axes.</li>
          <li>Provide a bullet point outline of data analysis methodology.</li>
        </ul>
      <li>Discussion</li>
        <ul>
          <li>What are the benefits and drawbacks of differential tracking?</li>
          <li>Include a thorough discussion of error sources.</li>
        </ul>
    </ol>
  </li>
</ol>