Optical Microscopy: Part 2 Report Outline

• Update the apparatus section of your report to reflect the changes you made in part 2.

4. Fluorescence imaging and flat-field correction
   1. Procedure
      • Document the samples you used and how you captured images (camera settings, software used, etc…)
   2. Data
   • Include raw images of the stained cell sample, reference image, and dark image.
   • It may be clearer to show the reference image as a surface plot.
   3. Analysis and Results
   • Plot intensity profiles of the reference, dark, and raw images on a single set of axes. (Use the improfile command in MATLAB.)
   • Use the reference and dark images to correct the raw image for nonuniform illumination and include the corrected image.
   • Include histograms of the sample, reference, dark, and corrected images on a single plot of log(count) vs intensity.
   • Document the steps you used to process the image.
   4. Discussion
   • How did your beam expander design affect your images?
5. Resolution
   1. Procedure
      • Document the samples you used and how you captured images (camera settings, software used, etc…)
   2. Data
   • Include an image of the PSF sample with the beads used for resolution measurement indicated.
   3. Analysis and Results
   • Report the resolution you measured. Make sure to include N and a measure of uncertainty.
   • Show sample Gaussian fits.
   • Include a thorough discussion of error sources.
   4. Discussion
   • Include a thorough discussion of error sources.
   • Compare the measured value to the theoretical value.