Difference between revisions of "Optical Microscopy: Part 2 Report Outline"
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Revision as of 04:23, 16 February 2014
- Update the apparatus section of your report to reflect the changes you made in part 2.
- Fluorescence imaging and flat-field correction
- Procedure
- Document the samples you used and how you captured images (camera settings, software used, etc…)
- Data
- Include raw images of the stained cell sample, reference image, and dark image.
- It may be clearer to show the reference image as a surface plot.
- Analysis and Results
- Plot intensity profiles of the reference, dark, and raw images on a single set of axes. (Use the improfile command in MATLAB.)
- Use the reference and dark images to correct the raw image for nonuniform illumination and include the corrected image.
- Include histograms of the sample, reference, dark, and corrected images on a single plot of log(count) vs intensity.
- Document the steps you used to process the image.
- Discussion
- How did your beam expander design affect your images?
- Procedure
