Difference between revisions of "Optical Microscopy: Part 2 Report Outline"

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(Created page with "* Update the apparatus section of your report to reflect the changes you made in part 2. <br /> <ol start="4"> <li>Fluorescence imaging and flat-field correction <ol> ...")
 
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         <ul><li>How did your beam expander design affect your images?</li></ul>
 
         <ul><li>How did your beam expander design affect your images?</li></ul>
 
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  </li>  <li>Resolution
 
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    <ol>
 
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      <li>Procedure
 
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        <ul><li>Document the samples you used and how you captured images (camera settings, software used, etc…)</li></ul>
 
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      </li>
 
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      <li>Data</li>
 
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        <ul>
 
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          <li>Include an image of the PSF sample with the beads used for resolution measurement indicated.</li>
 
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        </ul>
 
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      <li>Analysis and Results</li>
 
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        <ul>
 
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          <li>Report the resolution you measured. Make sure to include N and a measure of uncertainty.</li>
 
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          <li>Show sample Gaussian fits.</li>
 
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          <li>Include a thorough discussion of error sources.</li>
 
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        </ul>
 
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      <li>Discussion</li>
 
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        <ul>
 
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          <li>Include a thorough discussion of error sources.</li>
 
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          <li>Compare the measured value to the theoretical value.</li>
 
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        </ul>
 
 
     </ol>
 
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Revision as of 04:21, 16 February 2014

  • Update the apparatus section of your report to reflect the changes you made in part 2.


  1. Fluorescence imaging and flat-field correction
    1. Procedure
      • Document the samples you used and how you captured images (camera settings, software used, etc…)
    2. Data
      • Include raw images of the stained cell sample, reference image, and dark image.
      • It may be clearer to show the reference image as a surface plot.
    3. Analysis and Results
      • Plot intensity profiles of the reference, dark, and raw images on a single set of axes. (Use the improfile command in MATLAB.)
      • Use the reference and dark images to correct the raw image for nonuniform illumination and include the corrected image.
      • Include histograms of the sample, reference, dark, and corrected images on a single plot of log(count) vs intensity.
      • Document the steps you used to process the image.
    4. Discussion
      • How did your beam expander design affect your images?
 </li>

</ol>

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