Lab Manual: Atomic Force Microscopy (AFM)

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DNA Melting Apparatus

Objectives and Learning Goals

  • Learn function of the 20.309 AFM and the relationships between its components.
  • Understand how to extract quantitative information from this tool.
  • Use the AFM to estimate the value of a fundamental physical constant.
  • Possibly to take images, probe sample stiffness, and/or perform protein pulling.
  • Analyze sources of uncertainty and noise in the system that limit the accuracy of measurements.
==Roadmap Scenarios and Milestones==