Lab 1 Report -- Nathan S Lachenmyer

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Calibration

All calibrations and experiments done at 20 mW.

Position Calibration

1um cal.png calibration: 502463 Volts / m = 1.99 um / volt

Trap Stiffness Calibration

Method Trap Stiffness (pN / nm)
Stokes 2.26e-5 pN/nm
Equipartition Theorem 1.23e-5 pN/nm
PSD *Need Bandwidth of DAQ*

Experiments

E Coli

Ecoli.png The frequencies here are not properly calibrated until I get the DAQ bandwidth. There are obvious peaks at what are (now) 10, 20, and 40 Hz -- I think these are the harmonics of the e coli rotation, and once the abscissa is properly scaled will give the e coli rotation speed.

DNA Tethers

Dna raw.png