ESTORM

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20.345: Bioinstrumentation Project Lab

Contents

1 Introduction
2 Instrument
- 2.1 Laser
- 2.2 Structured Light
3 Software
4 Sample Prep
5 Results
6 References
- 6.1 Structured Illumination

Introduction

Instrument

Laser

Structured Light

DLP Pico Projector

Software

Sample Prep

Results

References

Structured Illumination

"Super-resolution video microscopy of live cells by structured illumination," P. Kner, B. B. Chhun, E. R. Griffis, L. Winoto, and M. G. L. Gustafsson: Nat. Methods 6, 339 (2009) Achieved 100-nm resolution at frame rates up to 11 Hz
"Subdiffraction scattered light imaging of gold nanoparticles using structured illumination," Bo-Jui Chang, Shiuan Huei Lin, Li-Jun Chou, and Su-Yu Chiang: Opt. Lett. 36, 4773-4775 (2011)3-D SIM using spatial light modulator
"Nonlinear structured-illumination microscopy: Wide-field fluorescence imaging with theoretically unlimited resolution," M.G.L. Gustafsson: Proc. Natl. Acad. Sci. USA 102, 13081–13086 (2005)Achieved 50-nm resolution by exploiting nonlinear emission rate near fluorophore saturation
"Surpassing the lateral resolution limit by a factor of two using structured illumination microscopy", M.G.L. Gustafsson: J. Microsc. 198, 82-87 (2000)First demonstration of super-resolution SIM

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