ESTORM

Introduction

Old Wiki

Instrument

Laser

Power Settings

Sample Prep

Protocol

References

Structured Illumination

• "Super-resolution video microscopy of live cells by structured illumination," P. Kner, B. B. Chhun, E. R. Griffis, L. Winoto, and M. G. L. Gustafsson: Nat. Methods 6, 339 (2009)

Achieved 100-nm resolution at frame rates up to 11 Hz

• "Subdiffraction scattered light imaging of gold nanoparticles using structured illumination," Bo-Jui Chang, Shiuan Huei Lin, Li-Jun Chou, and Su-Yu Chiang: Opt. Lett. 36, 4773-4775 (2011)

3-D SIM using spatial light modulator

• "Nonlinear structured-illumination microscopy: Wide-field fluorescence imaging with theoretically unlimited resolution," M.G.L. Gustafsson: Proc. Natl. Acad. Sci. USA 102, 13081–13086 (2005)

Achieved 50-nm resolution by exploiting nonlinear emission rate near fluorophore saturation

• "Surpassing the lateral resolution limit by a factor of two using structured illumination microscopy", M.G.L. Gustafsson: J. Microsc. 198, 82-87 (2000)

First demonstration of super-resolution SIM