Difference between revisions of "ESTORM"
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| + | [[Category:20.345]] | ||
| + | [[Category:20.345 Projects]] | ||
| + | [[Category:Spring 2012]] | ||
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==Introduction== | ==Introduction== | ||
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[https://docs.google.com/spreadsheet/pub?hl=en_US&hl=en_US&key=0AgYYi-6RgyHIdGtnUlE1M1BLUkhfX0ZqWnhFa2ROS1E&output=html Power Settings] | [https://docs.google.com/spreadsheet/pub?hl=en_US&hl=en_US&key=0AgYYi-6RgyHIdGtnUlE1M1BLUkhfX0ZqWnhFa2ROS1E&output=html Power Settings] | ||
| + | |||
| + | ===Structured Light=== | ||
| + | [http://www.ti.com/tool/dlp1picokit DLP Pico Projector] | ||
==Software== | ==Software== | ||
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===Structured Illumination=== | ===Structured Illumination=== | ||
| − | *[http://www.nature.com/nmeth/journal/v6/n5/abs/nmeth.1324.html P. Kner, B. B. Chhun, E. R. Griffis, L. Winoto, and M. G. L. Gustafsson | + | *[http://www.nature.com/nmeth/journal/v6/n5/abs/nmeth.1324.html "Super-resolution video microscopy of live cells by structured illumination," P. Kner, B. B. Chhun, E. R. Griffis, L. Winoto, and M. G. L. Gustafsson: Nat. Methods 6, 339 (2009)] Achieved 100-nm resolution at frame rates up to 11 Hz |
| − | *[http://www.opticsinfobase.org/abstract.cfm?URI=ol-36-24-4773 Bo-Jui Chang, Shiuan Huei Lin, Li-Jun Chou, and Su-Yu Chiang | + | *[http://www.opticsinfobase.org/abstract.cfm?URI=ol-36-24-4773 "Subdiffraction scattered light imaging of gold nanoparticles using structured illumination," Bo-Jui Chang, Shiuan Huei Lin, Li-Jun Chou, and Su-Yu Chiang: Opt. Lett. 36, 4773-4775 (2011)]3-D SIM using spatial light modulator |
| − | *[http://www.pnas.org/content/102/37/13081 | + | *[http://www.pnas.org/content/102/37/13081 "Nonlinear structured-illumination microscopy: Wide-field fluorescence imaging with theoretically unlimited resolution," M.G.L. Gustafsson: Proc. Natl. Acad. Sci. USA 102, 13081–13086 (2005)]Achieved 50-nm resolution by exploiting nonlinear emission rate near fluorophore saturation |
| + | *[http://web.pdx.edu/~larosaa/Applied_Optics_464-564/Projects_Presented/Projects-2008/Structured_Illumination_Microscopy/2000_Gustafsson_SURPASSIN-by_a_FACTOR_OF_TWOj.1365-2818.2000.00710.pdf "Surpassing the lateral resolution limit by a factor of two using structured illumination microscopy", M.G.L. Gustafsson: J. Microsc. 198, 82-87 (2000)]First demonstration of super-resolution SIM | ||
Latest revision as of 01:50, 28 February 2012
Contents
Introduction
Instrument
Laser
Structured Light
Software
Sample Prep
Results
References
Structured Illumination
- "Super-resolution video microscopy of live cells by structured illumination," P. Kner, B. B. Chhun, E. R. Griffis, L. Winoto, and M. G. L. Gustafsson: Nat. Methods 6, 339 (2009) Achieved 100-nm resolution at frame rates up to 11 Hz
- "Subdiffraction scattered light imaging of gold nanoparticles using structured illumination," Bo-Jui Chang, Shiuan Huei Lin, Li-Jun Chou, and Su-Yu Chiang: Opt. Lett. 36, 4773-4775 (2011)3-D SIM using spatial light modulator
- "Nonlinear structured-illumination microscopy: Wide-field fluorescence imaging with theoretically unlimited resolution," M.G.L. Gustafsson: Proc. Natl. Acad. Sci. USA 102, 13081–13086 (2005)Achieved 50-nm resolution by exploiting nonlinear emission rate near fluorophore saturation
- "Surpassing the lateral resolution limit by a factor of two using structured illumination microscopy", M.G.L. Gustafsson: J. Microsc. 198, 82-87 (2000)First demonstration of super-resolution SIM
