Difference between revisions of "DNA Melting: Simulating DNA Melting - Intermediate Topics"
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Begin by writing a function that will compute <math>\left . f \right .</math> from the equation derived [[DNA Melting Thermodynamics|in class]]. This function must be in its own file called DnaFraction.m. | Begin by writing a function that will compute <math>\left . f \right .</math> from the equation derived [[DNA Melting Thermodynamics|in class]]. This function must be in its own file called DnaFraction.m. | ||
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Revision as of 19:19, 10 April 2008
Write a function to compute f
Begin by writing a function that will compute $ \left . f \right . $ from the equation derived in class. This function must be in its own file called DnaFraction.m.
<include src="http://web.mit.edu/~20.309/www/Matlab/DnaFraction.m" />
%Returns the fraction of dsDNA given total DNA concentration, temperature, Delta S, and Delta H %Usage: f = DnaFraction(Ct, T, DeltaS, DeltaH) function f = DnaFraction(Ct, T, DeltaS, DeltaH) %Constants R=8.3; %first compute Ct * Keq CtKeq = Ct * exp(DeltaS / R - DeltaH / (R * T)); %now compute f f = (1 + CtKeq + sqrt(1 + 2 * CtKeq))/CtKeq;
Test the function
First, create a temperature vector. Then call DnaFraction with some reasonable parameters and plot the result. Units are calorie, mole.
t = [20:90] + 273; f = DnaFraction(.1E-6, t, 304E3, 786); plot(t,f)
