DNA Melting: Model function and parameter estimation by nonlinear regression
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Revision as of 17:32, 13 November 2012 by Steven Wasserman (Talk | contribs)
This subject matter will be explained in lecture, in tutorials, and in the lab.
Photobleaching model
Assumptions
- The dye is divided into two populations, bleached and unbleached, with concentrations S and S
- The initial dye concentration is S + S
- Bleached and unbleached molecules bind to dsDNA with the same affinity.
- Only bound dye molecules may be excited.
- An excited fluorophore will either bleach with probability p or return to the ground state with probability 1-p.
- Mechanisms for returning to the ground state include fluorescence, phosphorescence, and non-radiative relaxation.
- Only excited fluorophore molecules bleach
- Bleaching is irreversible