DNA Melting: DNA Sequences

From Course Wiki
Revision as of 18:28, 9 October 2013 by Eliot Frank (Talk | contribs)

Jump to: navigation, search

Single-stranded DNA sequences:

Name Sequence MW GC %
20bp ATC AAG CAG CCA TCG AAA CT 6079 45
20bp-comp AGT TTC GAT GGC TGC TTG AT 6154 45
20bp-single mismatch AGT TTC GAT TGC TGC TTG AT 6129 40
20bp-double mismatch AGT TTC GAT TGA TGC TTG AT 6129? 40?
20bp-complete mismatch CAC CGA CGT ACT ATT CCT AA 6021 45
30bp ATC AAG CAG CCA TCG AAA CTT AGG CTT ACA 9168 43.3
30bp-comp TGT AAG CCT AAG TTT CGA TGG CTG CTT GAT 9243 43.3
40bp ATC AAG CAG CCA TCG AAA CTT AGG CTT ACA ACC AGT GAC T 12242 45
40bp-comp AGT CAC TGG TTG TAA GCC TAA GTT TCG ATG GCT GCT TGA T 12348 45

For double-stranded DNA solutions, one of the 20bp, 30bp, or 40bp sequences is mixed in equal concentration with its respective complement, e.g., 30bp and 30bp-comp, in a desired saline solution. For 20bp mismatch solutions, the 20bp sequence is mixed in equal concentration with one of the mismatch sequences.